CORE--TISSUE CULTURE AND MONOCLONAL ANTIBODY

描述：(申请人描述)组织培养/克隆 抗体核心(TC/MB)在过去的12年里一直是一种有效的 UCCC调查人员访问专门细胞培养的资源 技术和生产单抗(MAb)。细胞融合和 自建立分泌单抗的杂交瘤细胞系以来， 该设施的建立并将继续是一项主要职能。生产 杂交瘤细胞在常规旋转容器中大规模生长产生的单抗 在中空纤维生物反应器中是最近建立的，该组件 杂交瘤服务在过去的四年中增长了大约300% 好几年了。杆状病毒载体的构建及重组表达 昆虫细胞中的蛋白质，在上一次审查中被确立为一项服务，已经 也显著增长(200-300%)，成为核心的主要功能。 TC/MB Core已经开发并维护了150多个存储库 液氮中的癌细胞株并为UCCC研究人员提供种子 用于实验的培养物或来自大容量培养物的细胞颗粒 用于分离大分子或其他细胞因子的来源。1997年，一个 成立了现场媒体供应中心，提供商业上的 准备好的液体生长介质、补充剂和血清 价格，并为UCCC调查人员节省了超过18万美元。除了……之外 技术服务，TC/MB核心提供咨询/培训和质量 控制程序。已使用一个或多个TC/MB核心服务 自上次审查TC/MB以来，UCCC调查人员117人。这些用户来自 90%的项目和超过95%的项目拥有同行评议的校外研究 资助金。定制技术服务已为超过 在过去的4年里，UCCC调查人员发表了180篇文章。未来的目标 TC/MB核心的核心是继续提供现有的优质服务和 扩大定制杆状病毒服务能力，规模化增长 增长最快的杂交瘤。要扩展这些设备的容量， 服务，要求每年小幅增加5.5%的核心赠款预算，因此 技术人员可以从3人增加到4人。另外， 第二年需要新设备。

Description: (Applicant's Description) The Tissue Culture/Monoclonal Antibody Core (TC/MB) has served for the past 12 years as an efficient resource for UCCC investigators to access specialized cell culture technologies and produce monoclonal antibodies (MAbs). Cell fusion and construction of MAb secreting hybridoma cell lines has been offered since the founding of the facility and continues to be a major function. Production of MAbs from large scale growth of hybridomas in conventional spinner vessels and in hollow-fiber bioreactors was established more recently and this component of hybridoma services has grown by approximately 300% over the past four years. Construction of baculovirus vectors and expression of recombinant proteins in insect cells, established as a service at the last review, has also grown significantly (200-300%) to become a major function of the Core. The TC/MB Core has developed and maintains a repository of more than 150 cancer cell lines in liquid nitrogen and provides UCCC investigators with seed cultures for experiments or cell pellets from large volume cultures as a source for isolation of macromolecules or other cellular factors. In 1997, an On-Site Media Supply Center was established that provides commercially prepared liquid growth medium, supplements and serum at substantially reduced prices and has saved UCCC investigators over $180,000. In addition to technical services, the TC/MB core provides consultation/training and quality control procedures. One or more of the TC/MB Core services has been used by 117 UCCC investigators since the last review of the TC/MB. The users are from 9 of 10 Programs and more than 95% hold peer-reviewed extramural research grants. The custom technological services have provided support for more than 180 publications of UCCC investigators over the last 4 years. The future goal of the TC/MB Core is to continue to provide existing quality services and expand the capacity of custom baculovirus services and large scale growth of hybridomas that have increased the most. To expand the capacity of these services, a small annual increase of 5.5% in Core grant budget is requested so that the technical staff can increase from 3 to 4 individuals. Additionally, new equipment is requested in the second year.