FLOW CYTOMETRY & CELL SORTING SHARED RESOURCE

FCCSSR提供多种基于细胞的研究服务，主要是分析和分选流式细胞术。高速细胞分析和分选所需的仪器设备价格昂贵，需要高水平的技术专业知识；因此，该设备最好位于机构共享资源中。FCCSSR规定了仪器的维护，共享资源的运行，以及在实验工作中使用流式细胞仪的培训和咨询。工作人员为教职员工、专业实习生和学生提供使用流式细胞仪所需的全部技能的指导和培训，包括实验设计， 样品准备和染色、数据采集、采集后分析和分类。工作人员定期为调查人员组织培训课程，由来访的技术专家或工作人员自己授课。共享资源提供与研究人员数据和方法有关的材料，可支持赠款申请的实验可行性，并在需要时提供基本数据的出版质量表示。这种共享资源的一个独特功能是纳入资源设计的开发工作。由于FCCSSR的工作人员和主任一直积极开发流式细胞仪的新技术，因此共享资源与 与公司合作开发荧光检测和仪器的新技术，并与其他研究人员合作开发新的生物信息学方法。这一功能将促进新协议和技术的快速采用。小詹姆斯·E·克罗博士。是流式细胞术和细胞分选共享资源的科学总监。他定期与员工会面，审查共享资源绩效、政策、账单和预算状态， 人事问题、方案和程序、质量保证和质量控制以及发展研究。

The Flow Cytometry and Cell Sorting Shared Resource (FCCSSR) offers a variety of cell based research services, principally analytical and sorting flow cytometry. The instrumentation necessary for high speed cell analysis and sorting is expensive and requires a high level of technical expertise; therefore, this equipment is best situated in an institutional Shared Resource. The FCCSSR provides for the maintenance of the instrumentation, operation of the Shared Resource, and training and consultation in the use of flow cytometry in experimental work. The staff offers guidance and training to faculty, staff, professional trainees and students in the entire range of skills needed to utilize flow cytometry, including design of experiments, sample preparation and staining, data acquisition, post-acquisition analysis, and sorting. The staff organizes regular training sessions for investigators, given by visiting technical specialists or by the staff themselves. The Shared Resource provides materials related to investigators' data and approaches that can support the feasibility of experiments for grant applications, and provides publication-quality representations of primary data when needed. One of the unique features of this Shared Resource is developmental work that is incorporated into the design of the resource. Because the personnel and directors of the FCCSSR have been active in development of new techniques in flow cytometry, the Shared Resource has relationships with corporations for the development of new techniques in fluorescence detection and instrumentation and with other investigators for novel bioinformatics approaches. This feature will facilitate rapid adoption of new protocols and techniques. Dr. James E. Crowe, Jr. is the Scientific Director of the Flow Cytometry and Cell Sorting Shared Resource. He meets regularly with staff to review Shared Resource performance, policies, billing and budget status, personnel issues, protocols and procedures, quality assurance and quality control, and developmental studies.