MOLECULAR AND EXPERIMENTAL PATHOLOGY CORE

芝加哥大学DDRCC的分子实验病理学中心为这项研究提供了至关重要的服务 肠道疾病，特别是免疫介导的、感染性的、肿瘤性的和炎性的肠道疾病， 和动物组织。简单来说，核心部分可分为四个主要方面：（1）人类和动物 （主要是小鼠）组织库，（2）解剖病理学审查和咨询以及组织分析（包括 免疫组织化学，激光捕获显微切割，组织阵列），和（3）动物模型的使用和设计指导 肠道疾病结节;（4）流式细胞术。分子实验病理学核心是通过创建， 2003年，对分子病理学核心进行了重组，以更好地满足DDRCC的需求。的 以前的核心提供专业分子技术和基因工程方面的培训和直接服务 体细胞和胚胎干（ES）细胞系，以及对人体组织和这些组织的遗传分析的有限获取。 然而，由于在上一个资助期间大学核心服务的重组， 芝加哥大学的转基因/敲除小鼠核心已经建立，科学的进步，许多 所提供的专门分子技术已经成为个人容易完成的常规方法， 研究者的实验室或通过其他芝加哥大学核心，例如DNA测序大会。DDRCC综述 成员决定最能支持和扩展其工作的服务，这导致了对访问的新关注 人类和动物组织，获得先进的工具，培训他们的使用，在数据解释的专业知识， 协助小鼠的使用，而不是开发。在可能的情况下，分子实验病理学核心 与现有资源合作，为核心成员和复员方案协调委员会成员节省了大量费用。 分子实验病理学中心的行政主任，Jerrold Turnet医学博士，哲学博士、监督 所有组件的操作，并直接参与所有人体组织相关的操作，提供解剖 病理学审查和咨询服务，并监督人类和动物组织免疫染色和组织阵列 生产分子实验病理学中心的联合主任，傅洋新博士，医学博士，哲学博士、导演 小鼠组织库，提供小鼠组织使用建议，提供动物模型使用和开发的直接帮助， 特别是IBD相关疾病，并监督流式细胞术服务的运作。

The Molecular Experimental Pathology Core of The University of Chicago DDRCC provides services vital to the study of intestinal disease, particularly immune-mediated, infectious, neoplastic and inflammatory bowel diseases, in human and animal tissues. In simplest terms, the core components can be broken into four major area:;: (1) human and animal (primarily mouse) tissue banking, (2) anatomic pathology review and consultation and tissue analysis (including immunohistochemistry, laser capture microdissection, tissue arrays), and (3) guidance in the use and design of animal nodels of intestinal disease and (4) flow cytometry. The Molecular Experimental Pathology Core was created through ,i reorganization of the Molecular Pathology Core in 2003 in an effort to better meet the needs of the DDRCC. The previous core provided training and direct services in specialized molecular techniques, genetic engineering of both iomatic and embryonic stem (ES) cell lines, and limited access to human tissues and genetic analysis of these tissues. However, due to restructuring of university core services during the previous funding interval, rbr example an excellent University of Chicago Transgenic/Knockout Mouse Core had been established, and advances in science, many of the specialized molecular techniques provided had become routine approaches easily accomplished in an individual investigator's lab or through other University of Chicago Cores, e.g. the DNA Sequencing Con;. A survey of DDRCC membership to determine the services that would best support and extend their work resulted ir a new focus on access to human and animal tissues, access to advanced tools, training in their use, expertise in data inrerpretation, and assistance in the use, rather than development, of mice. Where possible, the Molecular Experimental Pathology Core has partnered with existing resources to generate significant cost-savings to both the core and the DDRCC membership. The Administrative Director of the Molecular Experimental Pathology Core, Dr. Jerrold Turnet M.D., Ph.D., oversees the operations of all components and is directly involved in all human tissue-related operations, provides anatomic pathology review and consultation services, and supervises human and animal tissue immunostaining and tissue array production. The Co-Director of the Molecular Experimental Pathology Core, Dr. Yang-Xin Fu, M.D., Ph.D., directs, the mouse tissue bank, advises on mouse tissue use, provides direct assistance with use and development of animal models, particularly of IBD-related disease, and oversees the operation of flow cytometry services.