Campylobacter jejuni flagellar regulation and synthesis

空肠弯曲杆菌是许多动物胃肠道的肠道细菌，特别是 鸟然而，当C.空肠感染人类的胃肠道，这种细菌通常会导致 轻度到重度出血性结肠炎综合征。C.空肠目前被认为是食源性疾病的主要原因， 细菌性胃肠炎在世界范围内的人类。C.空肠已被确定为毒力 定植因子参与致病和定植的双重促进作用。然而，知识 关于C.与其他细菌相比，空肠是有限的。我们 发现C.空肠严格控制依赖于σ 54的鞭毛基因的转录， 鞭毛的重要组成部分本提案的总体目标是确定 关键鞭毛蛋白和调节蛋白的功能集中于控制依赖于σ 54的转录， 鞭毛基因本研究的中心假设是C.空肠协调地需要FlhF， 鞭毛输出器蛋白和FlgS/FlgR双组分转录调控系统 依赖于σ 54的基因对鞭毛运动至关重要。该项目的具体目标是：1） 评估FlhF在鞭毛基因调控和生物合成中的作用; 2）确定鞭毛组装如何 影响σ 54依赖性鞭毛基因的转录激活;和3）研究可能的 额外的层调控鞭毛基因转录和生物合成，并确定这些鞭毛 调节机制可能会影响鸟类的寄生虫病。实现这些目标将有助于：1） 了解关键鞭毛蛋白之间的联系，以及它们如何影响遗传机制， 控制鞭毛基因调控和生物合成; 2）增加我们对基因调控的理解 C.使用的途径空肠用于控制毒力和定殖因子的表达。 空肠弯曲杆菌在其表面产生一种称为鞭毛的结构， 运动性、促进人类的牙周病和动物的定植。拟议研究 活性将决定C. jejuni的某些蛋白质如何共同控制鞭毛的产生 构成鞭毛结构的蛋白质。

Campylobacter jejuni is a commensal bacterium of the gastrointestinal tracts of many animals, specifically birds. However, when C. jejuni infects the gastrointestinal tracts of humans, the bacterium often causes a mild to severe, bloody diarrheal syndrome. C. jejuni is currently recognized as a leading cause of food-borne bacterial gastroenteritis in humans world wide. The flagellum of C. jejuni has been identified as a virulence and colonization factor involved in promoting both pathogenesis and commensalism. However, knowledge regarding flagellar gene regulation and biosynthesis in C. jejuni is limited compared to other bacteria. We have found that C. jejuni tightly controls transcription of sigma 54-dependent flagellar genes encoding essential components of the flagellum. The overall objective of this proposal is to determine how the functions of key flagellar and regulatory proteins converge to control sigma 54-dependent transcription of flagellar genes. The central hypothesis of the proposed research is that C. jejuni coordinately requires FlhF, the flagellar export apparatus proteins, and the FlgS/FlgR two-component regulatory system for transcription of sigma 54-dependent genes essential for flagellar motility. The specific aims of this project are: 1) to assess the role of FlhF in flagellar gene regulation and biosynthesis; 2) to determine how flagellar assembly influences activation of transcription of sigma 54-dependent flagellar genes; and 3) to investigate possible additional layers of regulating flagellar gene transcription and biosynthesis and determine how these flagellar regulatory mechanisms may influence commensalism in birds. Accomplishment of these aims will aid in: 1) understanding connections between key flagellar proteins and how they influence genetic mechanisms to control flagellar gene regulation and biosynthesis; and 2) increase our understanding of genetic regulatory pathways used by C. jejuni for controlling expression of virulence and colonization factors. The bacterium Campylobacterjejuni produces a structure on its surface called a flagellum that is required for motility, promotion of diarrheal disease in humans, and colonization of animals. The proposed research activities will determine how certain proteins of C.jejunifunction together to control production of flagellar proteins that make up the flagellar structure.