Structural Components of the Campylobacter jejuni Polar Flagellar Motor

空肠弯曲杆菌极鞭毛马达的结构组件

• 批准号: 8428620
• 负责人: DAVID R HENDRIXSON
• 金额: $ 19.36万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-05 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8428620
• 关键词: Anabolism; Animals; Bacteria; Biochemistry; Biological Models; Birds; Campylobacter jejuni; Characteristics; Collaborations; Collection; Complex; Consumption; Country; Data; Disease; Electrons; Environment; Escherichia coli; Filament; Flagella; Foundations; Gastroenteritis; Genetic; Genetic Screening; Grant; Growth; Helicobacter; Homologous Gene; Human; Infection; Intestines; Investigation; Ions; Mastigophora; Meat; Medial; Membrane; Methods; Microscopy; Modeling; Molecular Biology; Molecular Machines; Motor; Movement; Mutagenesis; Nutrient; Organism; Peptidoglycan; Protein Biosynthesis; Protein C; Proteins; Pseudomonas; Research; Rotation; Salmonella; Salmonella enterica; Structure; Surface; Swimming; Techniques; Torque; Type III Secretion System Pathway; United States; Vibrio; Work; base; cell motility; extracellular; insight; nanomachine; novel; pathogen; pathogenic bacteria; periplasm; polymerization; programs; protein structure; public health relevance; research study; retinal rods; tomography

DESCRIPTION (provided by applicant): Flagellar motility is essential for many pathogenic bacteria to promote infection of hosts to result in disease. In addition, flagellar motility allows many environmental bacteria to reach specific niches and nutrients for optimal growth. The structure and function of a prototypic flagellar nanomachine is largely derived from cumulative studies of peritrichous flagella from Salmonella species and Escherichia coli. A typical flagellum is composed of a flagellar motor that extends from the inner and outer membranes, a surface-localized hook, and an extracellular filament that rotates. The flagellar motor is composed of stator complexes in the inner membrane that associate with rotor and switch components at the base of the flagellum and a rod that penetrates the peptidoglycan and the outer membrane. The stators generate an ion gradient to power the rotor to turn the rod and the filament, which results in swimming motility. The flagellar motors of polarly-flagellated bacteria contain these core components, but also possess additional structural components that are essential for motor function. By using electron cryo-tomography, we have identified three disk structures associated with the polar flagellar motor of Campylobacter jejuni, a leading cause of gastroenteritis in humans in the United States and in other countries throughout the world, which are essential for promoting motility. We have identified two proteins, FlgP and Cjj0413, which compose two of these motor disk structures. Deletion of either protein abolishes motor function and motility in C. jejuni. Because homologues of these proteins are found in other polar flagellates that also produce disk-like structures associated with their respective flagellar motors, we believe that we have identified components universally required for function of polar flagellar motors. The objectives of this proposal are to use C. jejuni as a model system to understand construction and function of flagellar motors as they relate to medically-important polar-flagellated bacterial pathogens such as Vibrio, Pseudomonas, and Helicobacter species. In Aim 1, we will perform experiments to identify other proteins of C. jejuni forming motor disk structures and proteins specifically required for flagellar motor function. In Aim 2, we will analyze essential residues of FlgP required for formation of the basal motor disk and nucleation of the other motor disks. In addition, we will analyze cytoplasmic and periplasmic domains of Cjj0413 and their requirements for polymerization of the proximal disk and motility. Accomplishment of these aims will aid in understanding: 1) unique components required for a functional polar flagellar motor; 2) alternative mechanisms for the construction and function of polar flagellar motors that differ from peritrichous motors; and 3) diversity amongst motile bacteria for how flagellar nanomachines function to promote swimming motility across bacterial species, which is essential for optimal growth of many bacteria in hosts and in environments.

描述（由申请人提供）：鞭毛运动是许多致病菌促进宿主感染而导致疾病的必要条件。此外，鞭毛运动允许