PATHOGENICITY OF SIVMAC239 VARIANTS IN NEONATAL MACAQUES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。目的：SIV和所有慢病毒都依赖于病毒Rev调节含RRE的mRNA的核质输出和表达。我们已经产生了SIV变体，其具有由D型逆转录病毒CTE的输出元件取代的基本病毒调节机制，CTE利用细胞因子hTAP进行其运输。被这些SIV变体感染的原代细胞产生较少量的具有降低的感染性的病毒。新生猕猴是评价潜在减毒SIV变体致病性的关键和敏感的模型系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: SIV and all lentiviruses depend on the viral Rev regulation for the nucleo-cytoplasmic export and expression of the RRE-containing mRNAs. We have generated SIV variants that have the essential viral regulatory mechanism replaced by the export elements of the type D retroviruses, CTE, which utilizes a cellular factor hTAP for its transport. Primary cells infected by these SIV variants produce lower amounts of virus having reduced infectivity. The neonate macaque represents a critical and sensitive model system to evaluate the pathogenicity of potentially attenuated SIV variants.