HUMAN PMNL MEMBRANE MICRODOMAINS AND THEIR MODIFICATIONS BY DEHP

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。以前的工作使用稳态荧光Laurden标记的PMNL悬浮液表明，塑料软化剂DEHP增加PMNL膜的流动性。特别是，该化合物降低了更刚性的膜域的序参数。本研究的目的是通过使用双光子显微镜观察单细胞上的膜流动性域，然后研究塑料软化剂DEHP如何影响它们。将通过Percoll梯度离心从人血中纯化PMNL，用DEHP孵育或不孵育，使其沉降到盖玻片上，用Laurdan标记，并在37 ℃下通过双光子显微镜分析Laurdan GP。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous work using steady-state fluorescence of Laurdan-labelled PMNL suspensions showed that the plastic softener DEHP increases PMNL membrane fluidity. In particular, the compound decreases the order parameter of the more rigid membrane domains. The aim of this study is to visualize membrane fluidity domains on single cells by using two-photon microscopy and then investigate how the plastic softener DEHP may affect them. PMNL will be purified from human blood by Percoll gradient centrifugation, incubated or not with DEHP, allowed to settle onto a glass coverslip, labelled with Laurdan and analysed for Laurdan GP by two-photon microscopy at 37¿C.