STUDY OF C ELEGANS BY FRET AND FCS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。利用FRET显微镜对C Elegans中整合素连接激酶（Integrin-Linked Kinase， ILK）蛋白相互作用进行了体内研究（Breusegem, et al 2002），结果支持使用FRET显微镜更详细地研究相互作用的可能性。在这项工作中，将继续使用FRET显微镜对成年线虫体内ILK相互作用的研究，并将使用FRET显微镜和荧光相关光谱检查ILK蛋白在胚胎状态下肌肉结构形成过程中的相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In vivo study of Integrin-Linked Kinase (ILK) proteins interactions in C Elegans using FRET microscopy was performed (Breusegem, et al 2002) and the results support the possibility of using FRET microscopy to study the interactions more in detail. In this work, the in vivo study of ILK interactions in adult C Elegans using FRET microscopy is continued and the interactions of the ILK proteins during the formation of muscle structure in the embryonic state will be examined using FRET microscopy and fluorescence correlation spectroscopy.