DISTRIBUTION AND CLEARANCE OF NASAL VACCINES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。体内显微镜中心分享了现有数据。 我们工作的目标是更好地了解鼻内喷雾（液体或干粉）接种疫苗的沉积部位及其清除率。将评价与经鼻接种疫苗的免疫原性增加或降低相关的各种疫苗制剂（液体和干粉），以确定疫苗沉积部位和清除率是否与疫苗的有效性/免疫原性相关。 影像学研究对这项工作至关重要。 基于诱导的抗原特异性免疫应答的大小，大型动物（豚鼠、兔、非人灵长类动物）的鼻免疫不如小鼠的鼻免疫有效。 我们的假设是，由于较大动物的无效疫苗递送，较大动物的鼻免疫不如在小鼠中观察到的有效。 我们希望确定鼻粘膜表面的鼻疫苗配方，并确定疫苗随时间的移动（粘液纤毛清除）。此外，我们想确定改变疫苗免疫原性的疫苗制剂是否会影响其体内分布和清除。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Center for In Vivo Microscopy shared existing data. The goal of our work is to better understand the site of deposition of vaccines administered nasally as a spray (liquid or dry powder) and their rate of clearance. Various vaccine formulations (both liquid and dry powder) that are associated with increased or decreased immunogenicity of the nasally administered vaccine will be evaluated to determine if the site of vaccine deposition and the rate of clearance are associated with the efficacy/immunogenicity of the vaccine. Imaging studies are crucial for this work. Nasal immunization of large animals (guinea pigs, rabbits, non-human primates) is not as effective as nasal immunization of mice based on the magnitude of the antigen-specific immune response induced. Our hypothesis is that nasal immunization of larger animals is not as effective as observed in mice due to ineffective vaccine delivery of larger animals. We would like to identify the nasal vaccine formula on the nasal mucosal surfaces and determine the movement of the vaccine over time (mucociliary clearance). Additionally, we would like to determine if vaccine formulations that alter the immunogenicity of the vaccine affect its distribution and clearance in vivo.