SYNAPTIC PLASTICITY IN THE MAMMALIAN BRAIN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。AMPA受体（AMPAR）进出突触的调节在突触发生、突触成熟和各种形式的突触可塑性中起着相关作用。目前用于特异性标记和研究AMPAR动力学的方法（免疫标记，电生理和生化测量，与荧光蛋白融合）在回答新合成的AMPAR亚基的位置和运输问题方面的能力有限。也很难确定ampar的合成是否发生在树突腔室的局部，如果是，这是否受到活性的调节。我们现在已经应用4Cys标签和FlAsH/ReAsH脉冲追踪标记来直接检查和比较特定亚细胞结构域中预先存在和最近合成的蛋白质的局部运输。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regulation of AMPA receptor (AMPAR) trafficking toward and away from synapses plays a relevant role in synaptogenesis, synapse maturation and various forms of synaptic plasticity. Current methods used to specifically label and study AMPAR dynamics (immunolabeling, electrophysiological and biochemical measurements, fusion with fluorescent proteins) are limited in their ability to answer questions on the location and trafficking of newly synthesized AMPAR subunits. It is also difficult to determine whether synthesis of AMPARs occurs locally in the dendritic compartment and, if so, whether this is modulated by activity. We have now applied 4Cys tags and FlAsH/ReAsH pulse-chase labeling for directly examining and comparing the local trafficking of preexisting and recently synthesized proteins in specific subcellular domains.