USE OF REASH FOR EM CONNECTIVITY STUDIES IN THE DROSOPHILA BRAIN

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们感兴趣的是评估是否双砷配体，特别是ReAsH，可用于研究果蝇的神经解剖学使用光转换和电子显微镜。在原理分析的证据，我们将评估是否一个四半胱氨酸基序修饰的突触结合蛋白特异性表达在一个确定的神经元亚类的苍蝇可以用来识别突触前的配置文件。这些结构已经在苍蝇。如果ReAsH/光转换在果蝇中起作用，我们将能够将修饰的转基因表达靶向许多已鉴定的神经元，这些神经元的突触连接的详细模式尚不清楚。这些研究将为研究果蝇大脑神经回路的发育和功能提供宝贵的资源。我们于2003年10月开始与NCMIR合作，与NCMIR的科学家交换了试剂和方案，目前正在解决苍蝇胚胎中ReAsH染色的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in assessing whether the biarsenical ligands, in particular ReAsH, can be used for studying neuroanatomy in Drosophila using photoconversion and electron microscopy. In proof of principle analysis we will assess whether a tetracysteine motif modified synaptotagmin protein expressed specically in one identified neuronal subclass in the fly can be used to identify presynaptic profiles. These constructs are already in flies. Should ReAsH/photoconversion work in the fly, we will be able to target expression of modified transgenes to many identified neurons whose detailed patterns of synaptic connectivity are not yet known. These studies would provide an invaluable resource for studying the development and function of neural circuits in the fly brain. We started our collaboration with NCMIR in October 2003, exchanged reagents and protocols with scientists at NCMIR and are currently trouble-shooting staining with ReAsH in fly embryos.