CRYSTAL COLLECTION OF BAK AND CCBL UBA DOMAIN

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Bcl-2同源拮抗剂杀手（Bak）是B细胞淋巴瘤（Bcl）蛋白家族的促凋亡成员，直接涉及许多与异常凋亡相关的病理（如癌症和神经退行性变）。任何与Bak相关的结构信息对于理解其功能作用至关重要，我们已经获得了先前的结构，并希望继续获得衍生化的Bak晶体，该晶体在我们的主场光源上衍射最多为3A。我们希望降低分辨率，以改善细化统计。cCBL和ccl -b是酪氨酸激酶受体（Met受体-癌症相关）的接头，其包含泛素E3连接酶结构域和c端UBA结构域。有趣的是，c-CBL UBA不结合泛素，而CBL-b UBA可以。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bcl-2 homologous antagonist killer (Bak) is a pro-apoptotic member of the B cell lymphoma (Bcl) family of proteins which is directly implicated in many pathologies related to aberant apoptosis (e.g. cancer and neurodegeneration). Any structural information related to Bak is crucial for the understanding of its functional role and we have obtained a previous structure and would like to follow up with a derivatized Bak crystal that diffracts on our home source at best at 3A. We hope to bring the resolution `down` to improve the refinement statistics. cCBL and CBL-b are adaptors for the tyrosine kinase receptors (Met receptors - cancer related), which contain a Ubiquitin E3 ligase domain and a C-terminal UBA domain. Interestingly, c-CBL UBA does not bind ubiquitin whereas the CBL-b UBA does.