NMR STUDIES ON BRAIN METABOLISM

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。正在使用双标记的13 C-14 N-谷氨酰胺来标记TCA循环代谢物（包括柠檬酸盐、富马酸盐和琥珀酸盐）以及TCA副产物（包括天冬氨酸盐、谷氨酸盐、苯丙氨酸和酪氨酸以及丙氨酸和乳酸盐），研究脑代谢。用多巴胺和多巴胺激动剂处理细胞以研究多巴胺对细胞中的细胞毒性事件的影响。瓜氨酸和精氨酸的额外测量将作为一氧化氮代谢的指示，并且用14 N标记嘌呤核苷酸（因为谷氨酰胺是这些核苷酸的直接前体）将使提取细胞中核苷酸的测量成为可能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brain metabolism is being studied using double labelled 13C-14N-glutamine to label TCA cycle metabolites including citrate, fumarate and succinate, in addition to TCA by-products including aspartate, glutamate, phenylalanine and tyrosine as well as alanine and lactate. Cells were treated with dopamine and dopamine agonits to study the effects of dopamine on citotoxic events in the cells. Additional measurements of citrulline and arginine will be performed as a indication of Nitric oxide metabolism and labeling of purine nucleotides with 14N (as glutamine is an immediated precursor of these nucleotides) will make possible the measurements of nucleotides in extracted cells.