USING HETEROLOGOUS TWO-HYBRID SCREENS TO EXPLORE PLASMODIUM PROTEIN-PROTEIN INT

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。双杂交筛选是探索蛋白质相互作用网络的有效方法。对于一些生物，如疟原虫，由于疟原虫基因组中AT-含量极高，使得双杂交文库的构建和筛选变得困难。我们将使用酵母双杂交文库来筛选与几个恶性疟原虫基因的相互作用，这些基因具有酵母同源基因，涉及一系列不同的细胞过程。然后，将克隆与已鉴定的酵母伙伴同源的疟原虫蛋白，并通过双杂交系统和生化方法测试其与疟原虫诱饵的相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Two-hybrid screening is a powerful method to explore protein-protein interaction networks. For some organisms such as Plasmodium, it is difficult to make and screen two-hybrid libraries due to extremely high AT-content of Plasmodium genome. We will use yeast two-hybrid libraries to screen for interactions with several P.falciparum genes which have yeast homologs implicated in an array of different cellular processes. Plasmodium proteins homologous to the identified yeast partners will then be cloned and tested for interaction with plasmodium baits in two-hybrid system and by biochemical methods.