GLOBAL ANALYSIS OF PROTEIN SUMOYLATION IN SACCHAROMYCES CEREVISIAE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。虽然SUMO对细胞因子的修饰是酿酒酵母中的一个重要过程，但底物的身份仍然很大程度上未知。使用基于质谱的方法，我们已经确定了271个新的SUMO目标。这些底物在多种生物过程中发挥作用，并极大地扩展了真核细胞中SUMO调控的范围。转录似乎是与新型SUMO底物的SUMO化相关的最普遍的过程，该SUMO底物存在于RNA聚合酶I、II和III、pol II转录延伸复合物以及各种染色质重塑、染色质修饰和染色质沉默复合物的基础转录机制中。此外，我们的全球分析揭示了一些有趣的生物模式中的SUMO目标，包括大分子复合物内的sumoylation目标的集群列表。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although the modification of cellular factors by SUMO is an essential process in Saccharomyces cerevisiae, the identities of the substrates remain largely unknown. Using a mass spectrometry-based approach, we have identified 271 new SUMO targets. These substrates play roles in a diverse set of biological processes and greatly expand the scope of SUMO regulation in eukaryotic cells. Transcription appears to be the most prevalent process associated with sumoylation with novel SUMO substrates found in basal transcription machinery for RNA polymerases I, II, and III, pol II transcriptional elongation complexes, and a variety of chromatin remodeling, chromatin modifying, and chromatin silencing complexes. Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes.