PROTEOMIC ANALYSIS OF MATURE MELANOSOMES FROM THE RETINAL PIGMENTED EPITHELIUM

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。用质谱仪分析视网膜色素上皮(RPE)中黑素小体的蛋白质含量。在样品制备的三种变体中，有两种发现有100多种蛋白质是共同的。检测到一些通常与其他细胞器相关的蛋白质。检测到几种溶酶体酶，免疫电子显微镜证实了组织蛋白酶D的存在，从而支持了先前提出的黑素体可能有助于摄取的光感受器外节段盘降解的观点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The protein content of melanosomes in the retinal pigment epithelium (RPE) was analyzed by mass spectrometry. More than 100 proteins were found to be common to two out of three variations of sample preparation. Some proteins normally associated with other organelles were detected. Several lysosomal enzymes were detected, with the presence of cathepsin D confirmed by immunoelectron microscopy, thus supporting the previously suggested notion that melanosomes may contribute to the degradation of ingested photoreceptor outer segment disks.