IDENTIFICATION OF DMC1 INTERACTING PROTEINS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。以前的生物化学特性的蛋白质组分的减数分裂重组复合物一直是有限的。重组复合物中只有少数组分被鉴定出来，并且这些组分仅在有丝分裂酵母或大肠杆菌中表达后的减数分裂背景之外进行了研究。杆菌我们正试图通过从正常细胞环境中纯化减数分裂重组复合物的成分来鉴定和表征它们的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous biochemical characterization of the protein components of meiotic recombination complexes has been limited. Only a few components of the recombination complex have been identified and these have been studied only outside of a meiotic context following expression in mitotic yeast or E. coli. We are attempting to identify and characterize components of the meiotic recombination complex by purifying them from the normal cellular context in which they function.