REGULATION OF THE IPL1/SLI15 COMPLEX

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。染色体必须准确地分离到子细胞，以防止基因组不稳定和非整倍体，这是所有肿瘤的标志。染色体的运动是通过微管与着丝粒的相互作用产生的，着丝粒是在着丝粒DNA上组装的专门的蛋白质复合物。准确的染色体分离取决于姐妹着丝粒双向定向，使它们结合到微管产生相反的纺锤体两极。尽管Ipl 1/Sli 15复合体检测并纠正动粒双取向的缺陷，但尚不清楚该复合体如何通过双取向的存在或不存在来调节。Ipl 1是一种由Sli 15蛋白激活的蛋白激酶。因此，我们将通过FRET分析Ipl 1和Sli 15在着丝粒上的相互作用，并测试这种相互作用是否因存在或不存在双向取向而改变。这些研究可能阐明有关Ipl 1/Sli 15复合物调控的重要细节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Chromosomes must accurately segregate to daughter cells to prevent genomic instability and aneuploidy, a hallmark of all tumors. Chromosome movements are generated through the interaction of microtubules with kinetochores, the specialized protein complexes that assemble on centromeric DNA. Accurate chromosome segregation depends on sister kinetochores biorienting such that they bind to microtubules arising from opposite spindle poles. Although the Ipl1/Sli15 complex detects and corrects defects in kinetochore biorientation, it is not clear how the complex is regulated by the presence of absence of biorientation. Ipl1 is a protein kinase that is activated by the Sli15 protein. We will therefore analyze the interaction between Ipl1 and Sli15 on kinetochores by FRET and test whether the interaction is altered by the presence or absence of biorientation. These studies may elucidate important details about the regulation of the Ipl1/Sli15 complex.