STRUCTURAL STUDIES OF WEST NILE ENVELOPE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。西尼罗河病毒是美国一种新出现的传染病的病原体，NIAID认为它是B类优先病原体。目前还没有针对西尼罗河病毒的许可疫苗或特定的抗病毒治疗方法；因此，迫切需要开发疫苗、化学预防或化疗药物来预防或治疗越来越多的确诊病例，药物治疗将是国家生物防御装备的重要组成部分。我们已经在旋转阳极发电机上结晶了西尼罗河包膜蛋白，并在100oK下收集了3.数据集，尽管低温条件很差。自那以后，我们已经改善了低温条件，目前正试图通过分子置换来解决相。我们希望提高SSRL的分辨率，并在必要时通过MAD/SAD实验获得相。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. West Nile virus is the etiological agent of an emerging infectious disease in the United States and is considered by the NIAID to be a category B priority pathogen. There is no licensed vaccine or specific antiviral therapeutic available for West Nile virus; therefore, the development of a vaccine, chemoprophylactic, or chemotherapeutic agent is urgently needed to prevent or treat a growing number of diagnosed cases and a drug treatment would be an important part of the nation's biodefense armament. We have crystallized the West Nile envelope protein and have collected a 3. dataset at 100oK, despite poor cryogenic conditions, on a rotating anode generator. We have since improved cryo conditions and are currently attempting to solve phases through molecular replacement. We hope to improve the resolution at SSRL, and if necessary, obtain phases through MAD/SAD experiments.