CRYSTAL STRUCTURES OF HUMAN ANNEXIN A2 IN COMPLEX WITH PHOSPHATIDYL-INOSITOL-4,5

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。膜联蛋白A2是钙和磷脂结合蛋白家族的一员，称为膜联蛋白。由于其与磷脂膜和细胞骨架结合的能力，该蛋白似乎在膜相关事件中发挥作用，如内吞作用、胞吞作用、膜/膜和膜/细胞骨架连接。最近的研究表明，膜联蛋白A2与含有磷脂酰肌醇-4，5-二磷酸的细胞膜部分特异结合。这种相互作用是磷脂酰肌醇-4，5-二磷酸的专一性的，而不是其他磷脂酰肌醇的。该络合物的离解常数在微摩尔范围内。我们纯化了重组人膜联蛋白A2，并在L-α-磷脂酰肌醇-4，5-二磷酸(仅脂的头基)存在下结晶。我们想要确定蛋白质复合体的高分辨率结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Annexin A2 is a member of the calcium- and phospholipid binding protein family called annexins. Due to its ability to bind to phospholipid membranes and the cytoskeleton, the protein seems to play a role in membrane-related events such as endocytosis, exocytosis, membrane/membrane and membrane/cytoskeleton linkage. It has been shown recently that annexin A2 specifically associates with parts of the cell membrane containing phosphatidyl-inosiol-4,5-biphosphate. The interaction is specific for phosphatidyl-inositol-4,5-biphosphate and not for other phosphoinositides. The dissociation constant for this complex is in the micromolar range. We have purified recombinant human annexin A2 and crystallized it in the presence of L-alpha-phosphatidylinositol-4,5-diphosphate (the headgroup of the lipid only). We would like to determine the high resolution structure of the protein complex.