SAXS STUDY OF THE LIGHT SENSING REACTION OF PHYTOCHROME

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。光敏色素是在植物和某些细菌中发现的光感受器，它们可以感知红光和远红光的持续时间和强度。根据接收到的光信号，光敏色素几乎控制或调节植物新陈代谢和发育的各个方面。初步的生化实验表明，光致变色蛋白的光激活引起了大量的域间运动，这是信号转导所必需的。为了研究这一重要信号反应的结构基础，我们将扩展Tokutomi等人早期的静态SAXS实验。(《二月》，第139-42页()。使用在SSRL的高级SAXS设施中可以获得的更高分辨率的数据，我们将能够生成更详细、更高保真的光敏色素模型。此外，我们将使用时间分辨SAXS研究结构域重排在光敏色素光激活信号反应中的作用

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phytochromes are photoreceptors found in plants and certain bacteria where they sense the duration and intensity of red and far-red light. According to the received light signals, phytochromes then control or modulate virtually every aspect of plant metabolism and development. Preliminary biochemical experiments suggest that light activation of phytochromes causes large inter-domain motions which are essential for signal transduction. To investigate the structural basis of this important signaling reaction, we will extend earlier static SAXS experiments by Tokutomi, et al. (FEBS 274, pp. 139-42 (1989). Using the higher resolution data obtainable at the superior SAXS facility at SSRL, we will be able to generate a more detailed, higher fidelity model of phytochrome. Additionally, we will investigate the role of domain rearrangements in the light-activated signaling reaction of phytochrome using time-resolved SAXS