STRUCTURE OF COPII COATED VESICLES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。真核细胞的内膜是一种动态的结构，通过特殊的细胞机制如外壳蛋白复合体II(COPII)的活动而保持持续的通讯，COPII介导内质网(ER)的货物输出。COPII由Sar1 GTPase Sec23/SEC24(Sec23/24)和具有结构作用的Sec13/Sec31(Sec13/31)组成，Sec23是Sar1特异性GTPase激活蛋白，SEC24参与货物选择。虽然最近的结果表明Sec23/24和Sec13/31可以自组装形成COPII笼状颗粒，但我们现在已经证明，在没有Sec23/24的情况下，Sec13/31可以自组装成最小的笼子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Endomembranes of eukaryotic cells are dynamic structures that are in continuous communication through the activity of specialized cellular machineries such as the coat protein complex II (COPII), which mediates cargo export from the endoplasmic reticulum (ER). COPII is comprised of the Sar1 GTPase, Sec23/Sec24 (Sec23/24), where Sec23 is a Sar1-specific GTPase activating protein and Sec24 functions in cargo selection, and Sec13/Sec31 (Sec13/31), which has a structural role. While recent results showed that Sec23/24 and Sec13/31 can self-assemble to form COPII cage-like particles, we have now shown that Sec13/31 can self-assemble to form minimal cages in the absence of Sec23/24.