DEVELOPMENT OF MR MICROCOILS FOR MICROIMAGING & SPECTROSCOPY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。微小样品的微成像和光谱学需要开发小型射频线圈，以便最大限度地获得信号。根据核心2的目标#1，我们一直在为750MHz仪器构建一系列螺线管线圈，用于从单细胞，海藻酸珠，脊髓，肌肉纤维和小鼠大脑等一系列样品，这项工作今年一直在继续。今年需要将灌注与探针结合，特别是与我们的合作者一起研究海藻酸珠和胰岛。此外，P31线圈已被构建用于分离肌纤维的研究。我们还构建了在4.7T， 11.1T和17.6T系统下运行的13C和O17线圈。13C线圈支持杜克大学Mike Gamscik博士对大鼠肿瘤光谱的研究。O17是与Wei Chen合作开发的，用于监测组织耗氧量。信噪比在这些研究中是至关重要的，我们把最大的希望寄托在17.6T的磁铁上。到目前为止，我们已经构建了一个具有O17线圈，单独的蝴蝶1H线圈以及动物维护和监测的竞争rf探针和探针体。使用该探针，我们获得了活体小鼠大脑的第一个O17 CSI数据和O17图像。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microimaging and spectroscopy of very small samples requires the development of small rf coils in order to maximize the signal obtained. In accordance with Aim #1 of Core 2, we have been constructing a range of solenoidal coils for the 750MHz instrument for a range of sample ranging from single cells, alginate beads, spinal cords, muscle fibers and mouse brains, and this work has continued this year. This year has required the incorporation of perfusion with the probes, particularly for studies with our collaborators on alginate beads and islets. Additionally P31 coils have been constructed for studies of isolated muscle fibers. We have also constructed coils for operation at 13C and O17 at 4.7T, 11.1T and on the 17.6T systems. The 13C coils support studies on spectroscopy of rat tumors with Dr Mike Gamscik (Duke). The O17 is being developed in collaboration with Wei Chen for monitoring tissue oxygen consumption rate. The SNR is critical in these studies, and we are pinning our best hopes on the 17.6T magnet. To date, we have constructed a compete rf probe and probe body with an O17 coil, a separate butterfly 1H coil, and animal maintenance and monitoring. Using this probe we have obtained the first O17 CSI data and O17 images of a live mouse brain.