NMR MICROSCOPY OF SINGLE NEURAL CELLS & BRAIN SLICES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。研究继续通过使用各种模型来探索组织中磁共振信号的基本来源，从灌流的隔离组织(脑和心脏)切片到隔离的单细胞和血细胞幽灵。我们的目标是确定在组织中观察到的多指数水扩散的起源，并预期这些信息可以导致对临床MRI中信号变化的定量了解。继续对神经组织扩散测量中的信号特征进行建模。最近，我们开始研究固定剂对这些测量的影响，因为众所周知，固定会使磁共振信号发生显著变化。这对于解释来自固定组织的信号并与活体数据进行比较非常重要。此外，我们已经开始研究温度对扩散曲线的影响，测量由我们的模型确定的ADC和汇率随温度的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Studies continue exploring the fundamental origins of MR signals in tissues by using a variety of models ranging from perfused isolated tissue (brain and heart) slices through isolated single cells and blood cell ghosts. The goal is to determine the origins of multiexponential water diffusion observed in tissues in the anticipation that this information can lead to a quantitative understanding of signals changes in clinical MRI. Modeling the signal characteristics in diffusion measurements in neural tissues continues. More recently we have begun to examine the effects of fixatives on these measurements, since it is well known that the MR signals change significantly with fixation. This is important for interpreting signals from fixed tissue and making comparisons with in vivo data. Additionally we have begun to examine the effect of temperature on the diffusion curves, measuring temperature dependant changes in the ADC and exchange rate as determined by our models.