MAGNETIC RESONANCE IMAGING OF GLUTATHIONE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。我们将测试谷胱甘肽对预测癌症治疗效果的有效性。麸质存在于所有组织中，并保护组织免受毒素的侵害。癌细胞利用谷胱甘肽的保护功能来保护细胞免受化疗的影响，因此对治疗有抗性的癌症通常显示出高水平的谷胱甘肽。对治疗的抵抗是治疗不能治愈癌症的主要原因。因此，数十项临床研究表明，肿瘤组织活检样本中的谷胱甘肽可能是化疗反应的重要预测因子。以前，我们使用质子去耦13 C NMR在4.7T，以证明这种方法的可行性，这些数据被用来成功获得R21资助，以支持这些研究。从那时起，我们一直在寻求改善信噪比，并在11.1T上实施了类似的程序。在线圈构造之后，遇到了相当多的问题，因为仪器没有针对13 C进行适当的配置。此外，13 C通道中存在显著噪声，使研究无效。这可以追溯到几个来源，最值得注意的是接收端的滤波器。在布鲁克的帮助下，特别是Mark Mattingly，该硬件已被修复或更换，我们的最新结果令人满意。 在初步测试中，在杜克制备的具有标记输注的小鼠已被离体检查，并提供了令人鼓舞的结果，其中信号测量具有足够的SNR以允许基本的1和2D光谱。我们正在准备进行体内研究

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will be testing the effectiveness of glutathione for predicting the effectiveness of cancer treatment. Glutathione is found in all tissue and protects the tissue from toxins. Cancer cells have used the glutathione protective function to shield cells from the effect of chemotherapy and thus cancers which are resistant to therapy often show high levels of glutathione. Resistance to therapy is the primary reason therapy fails to cure cancer. Therefore, dozens of clinical studies have shown that glutathione in tumor tissue biopsy samples may be an important predictor of response to chemotherapy. Previously we used proton decoupled 13C NMR at 4.7T to demonstrate the feasibility of this approach, and these data were used to successful obtain an R21 grant to support these studies. Since then we have been in search of improved SNR and have implemented similar procedures on the 11.1T. After coil construction considerable problems were encountered, since the instrument had not been appropriately configured for 13C. Additionally significant noise existed in the 13C channel making the studies ineffective. This has been traced to several sources, most notably the filters on the receiver side. With help from Bruker, Mark Mattingly specifically, this hardware has been fixed or replaced and our latest results are satisfactory. In a preliminary test, mice prepared at Duke with a label infusion have been examined ex vivo and provided encouraging results, with signals measured with enough SNR to allow rudimentary 1 and 2D spectroscopy. We are now preparing fro in vivo studies