The Role of Separase During Cytokinesis in C elegans

细胞分裂的过程需要各种细胞过程的正确顺序和时间安排，以确保 遗传物质和细胞内容的忠实传递。进入后期发生在 姐妹染色单体凝聚力被一种被称为分离酶的蛋白酶切割。Separase最近被 显示促进后期纺锤体动力学和胞质分裂伴随着染色体分离 在萌芽酵母中。我们实验室以前的研究发现了一种温度敏感的等位基因 分离线虫中的同源物。这些分离酶突变的蠕虫的胚胎转移到限制性 温度表现出几种减数分裂和有丝分裂缺陷，如染色体不分离、渗透和 机械敏感性和胞质分裂失败。利用各种遗传和细胞生物学技术，我 建议揭示分离酶促进细胞质分裂完成的机制。 线虫的早期胚胎。通过这种分析，我希望能扩展对分子的理解 染色体分离与后期开始的时间协调机制 和胞质分裂。

The process of cell division requires the correct sequence and timing of various cellular processes to ensure faithful transmission of the genetic material and cellular contents. The entry to anaphase occurs upon the cleavage of sister-chromatid cohesion by a protease known as separase. Separase has recently been shown to promote anaphase spindle dynamics and cytokinesis concomitantly with chromosome segregation in budding yeast. Previous studies from our laboratory identified a temperature-sensitive allele of the separase homologue in C. elegans. Embryos from these separase-mutant worms shifted to the restrictive temperature display several meiotic and mitotic defects such as chromosome nondisjunction, osmotic and mechanical sensitivity, and cytokinesis failure. Using various genetic and cell biological techniques, I propose to uncover the mechanisms by which separase contributes to the completion of cytokinesis in the early embryo of C. elegans. Through this analysis, I hope to extend the understanding of the molecular mechanisms utilized for the temporal coordination of chromosome segregation with the onset of anaphase and cytokinesis.