E6 Interaction with Cellular PDZ Proteins

描述（由申请方提供）：来自癌症相关人乳头瘤病毒（HPV）的E6癌蛋白与含有PDZ基序的细胞蛋白亚组相关。遗传学证据表明，E6与细胞PDZ蛋白的相互作用对于人类角质形成细胞中的完整HPV病毒复制和小鼠鳞状细胞中的转化表型都是必需的。由于细胞蛋白质的几种不同复合物通过PDZ相互作用与E6相互作用，因此未定义含有E6靶点的特定PDZ的意义。我们已经使用蛋白质组学方法来鉴定与癌症相关的E6癌蛋白相互作用的细胞蛋白质复合物。这已经鉴定了通过PDZ配体相互作用与E6相互作用的细胞蛋白质的新型复合物。本申请的目的是表征我们已经鉴定的PDZ复合物，并探索E6-PDZ蛋白相互作用的生物学意义。

DESCRIPTION (provided by applicant): E6 oncoproteins from cancer associated Human Papillomaviruses (HPV) associate with a subset of cellular proteins that contain PDZ motifs. Genetic evidence has shown that the interaction of E6 with cellular PDZ proteins is necessary for both full HPV virus replication in human keratinocytes and transformed phenotypes in mouse squamous cells. As several different complexes of cellular proteins interact with E6 via PDZ interactions, the significance of particular PDZ containing targets of E6 is not defined. We have used a proteomics approach to identify complexes of cellular proteins that interact with cancer associated E6 oncoproteins. This has identified novel complexes of cellular proteins that interact with E6 by PDZ ligand interactions. The purpose of this application is to characterize the PDZ complexes we have identified, and to explore the biological significance of E6-PDZ protein interactions.