IMMUNE MEDIATED ALTERATIONS IN CONNECTIVE TISSUE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。对患者和健康对照皮肤进行活检。1.成纤维细胞从活检组织培养用于体外代谢、分子和遗传研究。2.活检的皮肤切片是使用免疫细胞化学、原位杂交和PCR进行研究的。 获得血液样本用于评估在系统性硬化症中起作用的血清和细胞因子以及用于遗传免疫学研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biopsies of patient and healthy control skin are performed. 1. Fibroblasts are cultured from biopsied tissue for in vitro metabolic, molecular, and genetic studies. 2. Skin sections of biopsy are studies using immunocytochemistry, in situ hybridization, and PCR. Blood samples are obtained for assessment of serum and cellular factors playing a role in systemic sclerosis and for genetic immunologic studies.