DETERMINE VASCULAR RESISTANCE IN ARM DURING LBNP & FOREARM NEGATIVE PRESSURE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这是一项先导性研究，旨在确定肌源性张力与交感神经系统对骨骼肌血管床血管张力的影响。10名年龄在18岁至35岁之间的健康受试者将被招募接受程序，以测定在下半身负压和单臂局部负压时双臂的血管阻力。要检验的假设是，在肌源性(手臂负压)和交感神经活动(较低的身体负压)共同影响下，手臂的血管阻力将高于仅受交感神经影响(无负压)的手臂。受试者将按照既定的方法对他们的手臂和腿施加负压。心率和血压将通过手指袖口进行监测。将使用多普勒探头评估肱动脉的血流和直径，并在每只手臂放置静脉导管以监测去甲肾上腺素的水平。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a pilot study designed to determine the contribution of myogenic tone vs. sympathetic nervous system influences on vascular tone in skeletal muscle vascular beds. Ten healthy subjects ages 18 to 35 will be recruited to undergo procedures to determine vascular resistance in both arms during lower body negative pressure and localized negative pressure in one arm. The hypothesis to be tested is that vascular resistance will be higher in the arm under the influence of both myogenic (negative arm pressure) and sympathetic activity (lower body negative pressure) than the arm under sympathetic influence alone (no negative pressure). Subjects will have their arm and legs subjected to negative pressure according to established methods. Heart rate and blood pressure will be monitored by a finger cuff. Brachial artery flow and diameter will be assessed using a Doppler probe and intravenous catheters will be placed in each arm to monitor norepinephrine levels.