STANDARD VERSUS BIOFILM SUSCEPTIBILITY TESTING IN CYSTIC FIBROSIS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。患有囊性纤维化（CF）的人经常有肺部感染，这些感染反复发生或随着时间的推移而恶化。肺部感染是由细菌引起的。CF患者肺部最常见的细菌之一是铜绿假单胞菌（Pa）。抗生素治疗用于停止或减缓Pa的生长。为了帮助决定使用哪种抗生素，病人的痰液样本被送到实验室。实验室确定哪些抗生素似乎对治疗Pa感染最有效。这被称为抗生素敏感性测试。有时抗生素治疗并不像我们希望的那样有效。一个原因可能是肺中的Pa在一种称为生物膜的厚粘液中缓慢生长。我们目前测试抗生素敏感性的方法不能解释这种生物膜。然而，一种新型的抗生素敏感性测试，称为生物膜测试，已经开发出来。因为它更好地模拟了细菌在肺部的作用，所以在选择抗生素治疗CF患者的Pa肺部感染时可能更有用。本研究的目的是比较标准的抗生素敏感性试验与生物膜敏感性试验在CF和PA的人。参与者将根据其中一项敏感性测试的结果接受14天的抗生素治疗。使用哪种测试完全取决于偶然性。在治疗结束时，将测量肺中Pa的量，并且我们将观察通过生物膜敏感性选择的抗生素是否比通过标准敏感性测试选择的抗生素更多地减少肺中Pa的量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. People with cystic fibrosis (CF) often have lung infections, which occur repeatedly or worsen over time. The lung infections are caused by bacteria. One of the more common bacteria in the lungs of people with CF is Pseudomonas aeruginosa (Pa). Treatment with antibiotics is used to stop or slow down the growth of the Pa. To help decide which antibiotics to use, samples of patients sputum are sent to the laboratory. The laboratory determines what antibiotics appear to work best to treat the Pa infection. This is called antibiotic sensitivity testing. Sometimes the antibiotic treatment is not as effective as we would hope. One reason may be that Pa in the lungs grows slowly in a thick mucus called biofilm. The current ways that we test for antibiotic sensitivity do not account for this biofilm. However, a new type of antibiotic susceptibility testing, called biofilm testing, has been developed. Because it better mimics what the bacteria is doing in the lungs, it may be more useful in choosing antibiotics to treat Pa lung infections in patients with CF. The purpose of this study is to compare the standard antibiotic sensitivity test to the biofilm sensitivity test in people with CF and Pa. Participants will receive 14 days of antibiotic therapy, based on the results of one of the sensitivity tests. Which test is used is decided totally by chance. At the end of the therapy, the amount of Pa in the lung will be measured, and we will see if the antibiotics selected by the biofilm sensitivity reduced the amount of Pa in the lungs more than the antibiotics selected by the standard sensitivity test.