ROLE OF LEUKOTRIENE B4 METABOLISM IN SEVERE ASTHMA

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本研究探讨了CYP 4F 3对临床哮喘的潜在作用。将从哮喘和阻塞性疾病诊所招募40名重度或持续性哮喘患者，同时从整个社区招募40名对照。将抽取血液用于遗传学和白三烯代谢评价，并收集呼出气冷凝液用于NO和白三烯水平评价以及IgE和RAST检测。此外，对照受试者将完成问卷调查并接受肺功能测定。患者将接受4-6个月的临床、教育、生理和药理学评估和优化，包括高剂量的吸入性皮质酮和长效吸入性β受体激动剂，以最大限度地减少症状并优化肺功能，之后他们将再次访问GCRC进行血液和呼气冷凝物测试。在门诊GCRC访视期间，将要求护理人员进行问卷调查、获取知情同意书以及采集和处理血液样本。还要求提供生物统计支助，以协助分析样本功效。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study examines the potential contribution of CYP4F3 to clinical asthma. 40 patients with severe or persistent asthma will be recruited from the Asthma and Obstructive Disease clinics, while 40 controls will be recruited from the community at large. Blood will be drawn for genetics and evaluation of leukotriene metabolism, and the exhaled breath condensate will be collected for evaluation of NO and leukotriene levels, as well as IgE and RAST testing. In addition, the control subjects will complete a questionnaire and undergo spirometry testing. The patients will receive 4-6 months of clinical, educational, physiological, and pharmacological assessment and optimization, including high doses of inhaled corticosterones and long-acting inhaled beta-agonists to minimize symptoms and optimize lung function, after which they will again visit the GCRC for blood and exhale breath condensate testing. During the outpatient GCRC visits, nursing staff will be asked to administer the questionnaire, obtain informed consent, and draw and process blood samples. Biostatistics support is also requested to assist with the analysis of sample power.