Measuring biomolecular interactions using the Biacore T100

理解生物系统的关键是能够弄清楚不同的成分（例如蛋白质，DNA，小分子）如何相互作用。近年来，随着一种称为表面等离子体共振（SPR）的技术的发展，测量这种相互作用的方法取得了巨大进展。在过去的6年里，我们成功地采用了Biacore X仪器，这是一个基本的SPR设备，在各种系统中进行测量。然而，我们已经敏锐地意识到X的局限性。去年，Biacore推出了SPR技术的最新开发产品Biacore T100，它包含了比X更令人印象深刻的技术进步，使测量的灵敏度和复杂性大大提高。我们描述了植物和微生物科学的研究计划，将大大受益于收购的Biacore T100仪器。

Key to understanding biological systems is the ability to work out how different components (e.g. proteins, DNA, small molecules) interact with each other. Huge advances in measuring such interactions have occurred in recent years with the development of a technique called surface-plasmon resonance (SPR). For the past 6 years we have successfully employed a Biacore X instrument, which is a basic SPR apparatus, to make measurements in a variety of systems. However, we have become acutely aware of the limitations of the X. Last year Biacore launched its latest development in SPR technology, the Biacore T100, which contains impressive technological advances over the X, enabling measurements of greatly increased sensitivity and sophistication. We describe research programmes in plant and microbial sciences that will benefit enormously from the acquisition of a Biacore T100 instrument.