ENERGY METABOLISM IN METFORMIN-TREATED OBESE ADULTS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们建议在糖尿病前期(空腹血糖100-125 mg/dL)和肥胖(BMI 30-39 kg/m2)的一小群成年志愿者中进行一项开放标记的二甲双胍试验，以验证我们的主要假设：二甲双胍在基线和体力活动期间导致EE显著增加。我们的第二个假设是，EE的变化与骨骼肌中AMPK的激活之间存在关联。具体目标：1.准确测量二甲双胍治疗前后(4周)的全身能量消耗。2.评价肌肉AMPK活性在能量消耗中的作用。3.评估食物摄入量、身体成分和自由生活的体力活动。4.将总能量平衡(EE和饮食)的变化与临床指标如糖化血红蛋白、胰岛素、血糖、瘦素和儿茶酚胺相关联。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to conduct an open-label metformin trial in a small group of adult volunteers who are pre-diabetic (fasting glucose 100-125 mg/dL) and obese (BMI 30-39 kg/m2) to test our primary hypothesis: metformin induces a significant increase in EE at baseline and during physical activities. Our secondary hypothesis is that there are associations between the changes in EE and the activation of AMPK in the skeletal muscle. Specific Aims: 1. To accurately measure whole-body energy expenditure before and after metformin treatment (4 weeks). 2. To assess the role of muscle AMPK activities in energy expenditure. 3. To assess food intake, body composition, and free-living physical activity. 4. To correlate changes in overall energy balance (EE and diet) with clinical markers such as HbA1c, insulin, glucose, leptin, and catecholamines.