COBRE: UVM MED: CORE C: CELLULAR & MOLECULAR

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。佛蒙特州大学神经科学科布雷细胞和分子生物学核心设施为希望在研究中利用细胞和分子生物学的研究人员提供设备和专业知识，但他们可能没有必要的经验或设备。该设施包括以下主要设备：赛弗吉SELDI-TOF质谱仪、蔡司掌上激光显微解剖系统、应用生物系统7500FAST定量聚合酶链式反应系统、奥德赛红外成像仪、STORM磷成像仪、BioRad 2-D凝胶系统和Beckman超速离心机。该设施还配备了标准的分子生物学实验设备，包括3台Techne热循环仪、1台Techne梯度循环仪、分光光度计、光度计、细胞培养罩、孵化器和显微镜、蔡司荧光显微镜、低速离心机、摇动孵化器和其他几个孵化器。该设备用于各种各样的项目，在报告所述期间有40名教职员工、6名博士后助理、8名研究生和3名本科生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Vermont Neuroscience COBRE Cell and Molecular Biology Core facility provides equipment and expertise for researchers wishing to utilize cell and molecular biology in their research, but who may not have the experience or equipment necessary. The facility contains the following major pieces of equipment: Ciphergen SELDI-TOF Mass Spectrometer, Zeiss Palm Laser Microdissection System, Applied Biosystems 7500FAST Quantitative PCR System, Odyssey Infrared Imager, STORM Phosphoimager, BioRad 2-D Gel System and Beckman Ultracentrifuges. The facility is also fully equipped with the standard molecular biology laboratory equipment including: 3 Techne thermal cyclers, 1 Techne gradient cycler, spectrophotometer, luminometer, cell culture hood, incubators and microscopes, Zeiss fluorescence microscope, low-speed centrifuges, shaking incubator, and several other incubators. This equipment is used on a wide variety of projects, with a user base during the reporting period of 40 faculty, 6 postdoctoral associates, 8 graduate students and 3 undergraduate students.