COBRE: UNR: MOLECULAR BIOLOGY CORE (B)

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。核心B是分子生物学核心。该核心将通过提供重组蛋白的一般克隆和表达，重组腺病毒的生产，南部，北部和西部分析服务于所有核心项目。此外，Core B将设计用于实时PCR和DNA序列分析的引物和探针，以及共同免疫沉淀分析的开发。此外，该核心将执行COBRE项目所需的所有准备工作，用于后续的蛋白质分析和鉴定以及蛋白质分析。内华达大学有一个完善的蛋白质组学设施，内华达蛋白质组学中心，可以充分执行所有必要的蛋白质分析，如蛋白质质量和序列测定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core B is the Molecular Biology Core. This core will serve all of the cobre projects by providing general cloning and expression of recombinant proteins, recombinant adenovirus production, Southern, Northern and Western analysis. In addition, Core B will design primers and probes for real-time PCR and DNA seqeuence analysis along with co-immunoprecipitation assay development. In addition, this core will perform all preparative work required by the COBRE projects for the subsequent analysis and identification of proteins and protein profiling. The University of Nevada has a well-established proteomics facility, the Nevada Proteomics Center, that can adequately perform all the necessary protein analysis such as protein mass and sequence determination.