COBRE: UNR: MOLECULAR BIOLOGY CORE (B)

COBRE：UNR：分子生物学核心 (B)

• 批准号: 7720387
• 负责人: Cherie Singer
• 金额: $ 21.43万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720387
• 关键词: Adenoviruses; Centers of Research Excellence; Co-Immunoprecipitations; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Grant; Institution; Molecular Biology; Nevada; Polymerase Chain Reaction; Production; Protein Analysis; Proteins; Proteomics; Recombinant Proteins; Recombinants; Research; Research Personnel; Resources; Sequence Determination; Source; Time; United States National Institutes of Health; Universities; Work; assay development; design; expression cloning

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core B is the Molecular Biology Core. This core will serve all of the cobre projects by providing general cloning and expression of recombinant proteins, recombinant adenovirus production, Southern, Northern and Western analysis. In addition, Core B will design primers and probes for real-time PCR and DNA seqeuence analysis along with co-immunoprecipitation assay development. In addition, this core will perform all preparative work required by the COBRE projects for the subsequent analysis and identification of proteins and protein profiling. The University of Nevada has a well-established proteomics facility, the Nevada Proteomics Center, that can adequately perform all the necessary protein analysis such as protein mass and sequence determination.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 核心B是分子生物学核心。 该核心将通过提供重组蛋白的一般克隆和表达、重组腺病毒生产、Southern、北方和Western分析来服务于所有cobre项目。 此外，Core B将设计用于实时PCR和DNA序列分析的引物和探针，沿着免疫共沉淀试验开发。 此外，该核心将执行COBRE项目所需的所有准备工作，用于随后的蛋白质分析和鉴定以及蛋白质谱分析。 内华达州的大学有一个完善的蛋白质组学设施，内华达州蛋白质组学中心，可以充分执行所有必要的蛋白质分析，如蛋白质质量和序列测定。