NOVEL 2D,3D MORPHOMETRIC INDICATORS IN DYSPLASIA IN BARRETT'S ESOPHAGUS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Sabo博士于2005年12月获得了基于竞争性RFA的COBRE资助。该基金于2006年2月启动。 背景：Barrett食管上皮异型增生的分级为治疗决策提供了依据。评级遭受相当大的观察者间的差异。我们开发了一个客观的形态学模型来区分发育不良的等级（结果在USCAP 2006会议上发表）。新目标：1.将Barrett食管患者从异型增生进展为浸润性癌的时间与形态测量结果相关联。我们的初步研究表明，纹理最好区分发育不良等级。由于纹理反映染色质分布，我们假设量化纹理可能有助于预测从异型增生到浸润性癌的进展。2.利用激光捕获显微切割技术和基因芯片技术，将细胞的形态计量学模型与基因表达谱进行关联。当基因签名将与形态学相关时，可以开发具有预测和/或治疗潜力的新分子标记物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Sabo was awarded COBRE funding based on a competitive RFA in Dec. 2005. The funding was activated in Feb. 2006. Background: Grading of epithelial dysplasia in Barrett¿s esophagus provides the basis for treatment decisions. Grading suffers from considerable interobserver variability. We developed an objective morphometric model to differentiate between grades of dysplasia (results presented at USCAP 2006 conference). New goals: 1.To correlate the morphometrical results with time of progression from dysplasia to invasive carcinoma in Barrett¿s esophagus patients. Our preliminary study showed texture to best differentiate between dysplasia grades. Since texture reflects chromatin distribution, we hypothesized that quantifying texture may help to predict progression from dysplasia to invasive carcinoma. 2.To correlate between the morphometrical model, and the genetic profile of these cells, using laser capture microdissection and gene microarray technology. When gene signatures will be correlated with morphometry, novel molecular markers with predictive and or therapeutic potentials may be developed.