COBRE: U NEL: CORE A: METABOLOMICS: PROTEOMICS, MICROSCOPY, GENOMICS, CYTOMETRY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。虽然在过去的几年里，已经见证了一个爆炸的活动，挖掘嵌入在基因组序列中的信息，生物信息学，转录组学和蛋白质组学的方法，代谢组学分析的状态是在其起步阶段。在过去的几年里，UNL和UNMC在开发蛋白质组学，微阵列和生物信息学核心设施方面取得了重大进展，这些设施被氧化还原生物学中心（RBC）的成员使用。为了支持UNL和UNMC研究团体对大规模代谢分析系统日益增长的兴趣，并补充现有的蛋白质组学和转录组学水平的全球分析设施，我们投资了质谱平台，以支持高通量代谢组学分析。代谢组学设施由Ashraf Raza博士监督，目前他正在开发代谢组学，代谢分析和代谢指纹的方法。目前的项目包括，识别和定量小鼠肝细胞中的含硫代谢物，并跟踪人类单核细胞在分化为巨噬细胞期间分泌的花生四烯酸衍生代谢物的变化。除了这些努力，质谱设施还支持翻译后修饰的研究，研究蛋白质结构变化，蛋白质组学和其他一般应用。代谢组学RBC质谱核心设施位于比德尔中心，该中心设有UNL蛋白质组学，显微镜和基因组学核心设施。新的代谢组学RBC质谱核心设施占地约500平方英尺。RBC质谱核心设施拥有来自Applied Biosystems的QStar XL和Qtrap 2000仪器。该设施具有使用纳米或微米流速技术进行MS、LCMS、LCMS/MS和LCMS/MS/MS分析的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. While the past few years have witnessed an explosion of activity in mining information embedded in genomic sequences by bioinformatic, transcriptomic and proteomic approaches, the state of metabolomics analysis is in its infancy. In the past couple of years, UNL and UNMC have made significant strides in developing proteomics, microarray and bioinformatics core facilities, which are used by members of the Redox Biology Center (RBC). To support the increasing interest of the research communities at UNL and UNMC in large-scale metabolic profiling systems and to complement the existing facilities for global analysis at the proteomic and transcriptomic levels, we have invested in the mass spectroscopy platforms to support high throughput metabolomics analyses. The metabolomics facility is overseen by Dr. Ashraf Raza, currently he is developing methods for metabolomics, metabolic profiling and metabolic fingerprinting. Current projects includes, identifying and quantitating sulfur containing metabolites in mouse liver cells and tracking changes in arachidonic acid derived metabolites secreted by human monocytes during differentiation to macrophages. Besides these efforts, the mass spectrometry facility is also supporting research on post translational modifications, studying protein structural changes, proteomics and other general applications. The Metabolomics RBC Mass Spec Core Facility is located in the Beadle Center which houses the UNL Core Facilities for Proteomics, Microscopy and Genomics. The new Metabolomics RBC Mass Spec Core Facility occupies approximately 500 sq ft. RBC Mass Spec Core Facility has QStar XL and Qtrap 2000 instruments from Applied Biosystems. This facility has capabilities for doing MS, LCMS, LCMS/MS and LCMS/MS/MS analysis using nano or micro flow rate techniques.