COBRE: U NEL: CORE A: METABOLOMICS: PROTEOMICS, MICROSCOPY, GENOMICS, CYTOMETRY

COBRE：U NEL：核心 A：代谢组学：蛋白质组学、显微镜检查、基因组学、细胞计数

• 批准号: 8168305
• 负责人: Nandakumar Madayiputhiya
• 金额: $ 30.03万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168305
• 关键词: Animals; Area; Bioinformatics; Biological Markers; Biology; Centers of Research Excellence; Cholesterol; Complement; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cytometry; Dedications; Development; Dictionary; Disease; Ensure; Faculty; Fees; Funding; Genes; Genetic; Genomics; Glucose; Grant; Institution; Investigation; Lipids; Mass Spectrum Analysis; Metabolic; Metabolism; Microscopy; Modeling; Nebraska; Nutrient; Organism; Oxidation-Reduction; Peptides; Plants; Proteomics; Relative (related person); Research; Research Personnel; Resources; Source; Structure; System; Techniques; United States National Institutes of Health; Universities; base; cost; environmental change; expiration; instrumentation; member; metabolomics; microbial; programs; response; small molecule; transcriptomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metabolomics is the study of the repertoire of non-proteinaceous, endogenously-synthesized small molecules present in any organism. Small molecules present in the metabolome are ultimately the product of cellular metabolism. These molecules are varied from peptide, glucose, cholesterol, ATP and lipids etc. The change in levels of these metabolites in consequence of certain metabolic process or disease state and can be used as biomarkers. The power of metabolomic investigations versus transcriptomic and proteomic ones is that the metabolite dictionary is believed to be an order of magnitude smaller than the repertoire of expressed genes in an organism. In addition, metabolite profiles provide direct versus inferred readouts on the functional consequences of genetic and environmental changes. Furthermore, small metabolites structure is independent of the species under investigation and can be easily relate to different species. Recent developments at the University of Nebraska have led to the advent of genomics, bioinformatics and proteomics supported by dedication of faculty lines and creation of related core facilities at both UNL and UNMC. Therefore, we have developed a new capacity in the complementary area of metabolomics, which is at the cutting edge of functional biology research and is important for the programs of a number of investigators in the Redox Biology Center as well as for the other related research labs at UNL. This facility is complementing the existing infrastructural support in Mass Spectrometry (UNL), Proteomics (UNL) and Microarray (UNMC) analyses at the two institutions. In addition, UNL has well-equipped and staffed core facilities for genomics, microscopy and bioinformatics. Cellular responses to redox perturbations resulting from genetic, nutrient or environmental changes and can be analyzed in microbial, plant and animal systems at metabolic level. In projects being investigated by members of the Redox Biology Center, a number of potential projects need specialized mass spectrometry technique. The growing number of researchers associated with the Redox Biology Center need access to the instrumentation lab that supports metabolite profiling. In addition, the relative novelty of this capability will poise Nebraska to potentially tap into customer bases for other institutions in the geographic vicinity. Implementation of a nominal user fee system will recover costs for operating the facility after the expiration of the COBRE grant and ensure its long-term sustainability. Other core facilities at UNL and UNMC operate on a similar model.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 代谢组学是对任何生物体中存在的非蛋白质类、内源性合成的小分子的研究。代谢组中的小分子最终是细胞新陈代谢的产物。这些分子包括多肽、葡萄糖、胆固醇、三磷酸腺苷和脂类等。这些代谢物在一定的代谢过程或疾病状态下水平的变化，可作为生物标志物。与转录组和蛋白质组学研究相比，代谢组研究的力量在于代谢物词典被认为比生物体中表达的基因库小一个数量级。此外，代谢物图谱提供了关于遗传和环境变化的功能后果的直接和推断读数。此外，小的代谢物结构与所研究的物种无关，可以很容易地与不同的物种相关。内布拉斯加大学最近的发展导致了基因组学、生物信息学和蛋白质组学的出现，并在UNL和UNMC建立了相关的核心设施。因此，我们在代谢组学的互补领域开发了一种新的能力，这是功能生物学研究的前沿，对氧化还原生物学中心的一些研究人员的计划以及UNL的其他相关研究实验室都很重要。 这一设施正在补充这两个机构在质谱学(UNL)、蛋白质组学(UNL)和微阵列(UNMC)分析方面的现有基础设施支持。此外，UNL拥有完善的设备和人员配备的基因组学、显微镜和生物信息学的核心设施。细胞对遗传、营养或环境变化引起的氧化还原扰动的反应可以在代谢水平上在微生物、植物和动物系统中进行分析。在氧化还原生物中心成员正在调查的项目中，一些潜在的项目需要专门的质谱学技术。越来越多与氧化还原生物中心相关的研究人员需要进入支持代谢物分析的仪器实验室。此外，这种能力的相对新颖性将使内布拉斯加州有可能为地理附近的其他机构挖掘客户基础。实施象征性的使用费制度将在科布雷赠款到期后收回设施的运营成本，并确保其长期可持续性。后勤基地和军事中心的其他核心设施也采用类似的模式运作。