GENETICS OF THE SELF-INCOMPATIBILITY (S-) LOCI

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我已经完成了理论研究，产生了一种新的方法来创建自交不亲和（S-）位点附近的基因连锁图谱，这在以前是不可能的，因为该区域周围的重组受到抑制。基于聚结的新方法为研究抑制重组的基因组区域如何进化提供了新的工具。（我已经提交了一篇论文——Takebayashi， N.， D. E. Wolf，和L. F. Delph，异族通婚对Gilia achilleifolia种群内异交的影响）。目前，我们关注的是s位点进化的实证方面。2004年7月，我与Dr. Diana Wolf， UA Fairbanks合作提交了一份NSF提案（Papaveraceae自交不亲和位点的分子群体遗传学）。植物的自交不亲和性经过了多次独立进化。这种平行进化提供了一个难得的机会来确定哪些进化特征是普遍的，反复发生的，哪些特征仅仅是偶然的，或者是由于每个物种的特定特征而进化出来的。我们前期的研究发现，茄科植物的s位点呈现出一种意想不到的谱系形态，这可能是由于有害突变在非重组区积累所致。阿拉斯加罂粟与茄科SI系统具有完全不同的分子机制，本研究拟开展其s位点的分子群体遗传学研究。这是一个具有挑战性的项目，但将在概括包括y染色体在内的受抑制重组区域的进化过程方面取得重大突破。这个项目没有得到资助。在INBRE种子基金的帮助下，我们找到了几个阿拉斯加罂粟种群，并从几个种群中收集了种子。我们开始提取RNA，为重新提交提案收集初步数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I have completed theoretical investigations that resulted in a novel method to create a linkage map of genes near self-incompatibility (S-) locus, which was previously impossible due to suppressed recombination around the region. The new coalescent-based method provided a new tool for investigating how the genomic region of suppressed recombination evolved. (I have submitted a paper--Takebayashi, N., D. E. Wolf, and L. F. Delph, Effect of variation in herkogamy on outcrossing within a population of Gilia achilleifolia). Currently, we are focusing on empirical side of S-locus evolution. In July 2004, I submitted an NSF proposal (Molecular population genetics of the self-incompatibility locus in Papaveraceae) in collaboration with Dr. Diana Wolf, UA Fairbanks. Self-incompatibility (SI) in plants has evolved independently multiple times. This parallel evolution offers a rare opportunity to determine what evolutionary features are general, and occur repeatedly, and what features are simply due to chance or due to specific features of each species in which the trait evolved. Our previous studies found that S-locus of Solanaceae showed an unexpected genealogical shape, which may be due to accumulation of deleterious mutations in the non-recombining region. We proposed to initiate a study of the molecular population genetics of the S-locus in Alaskan poppies, which have a completely different molecular mechanism from Solanaceae SI system. This is a challenging project, but will allow a large break-through in generalizing the evolutionary process of regions under suppressed recombination including Y-chromosome. The project was not funded. With the aid of INBRE seed grant, we have located several populations of Alaskan poppies, and collected seeds from several populations. We are beginning RNA extractions to collect preliminary data for the resubmission of the proposal.