PUI RESEARCH-TOUGALOO-MAO

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在萌芽酵母中，大规模的基因缺失分析表明，在~6200个酵母基因中，超过80%是非必需的，这意味着真核细胞中的许多基因和途径必须是功能冗余的或在遗传扰动后的表型后果中得到缓冲。我对探索这种功能冗余很感兴趣，特别是当它与转录调控网络有关时。许多未知的开放阅读框架(ORF)与已知的转录因子家族具有序列同源性。使用高通量遗传分析(合成遗传阵列分析，或SGA)、DNA微阵列和全基因组定位分析或芯片上的分析，我想将这些未知的假定调控基因中的一个或多个与新的或现有的生理意义上的途径联系起来。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the budding yeast Saccharomyces cerevisiae, large-scale gene deletion analysis has shown that over 80% of the ~6200 yeast genes are non-essential, implying that many genes and pathways in eukaryotic cells must be functionally redundant or buffered from phenotypic consequences following genetic perturbation. I am interested in exploring this functional redundancy, especially as it relates to transcriptional regulatory networks. A number of uncharacterized open reading frames (ORFs) have sequence homology to well known families of transcription factors. Using high-throughput genetic analysis (Synthetic Genetic Array Analysis, or SGA), DNA microarrays, and Genome-wide location analysis or Chip on chip, I would like to connect one or more of these uncharacterized putative regulatory genes with a new or existing physiologically significant pathway.