LOUISIANA COBRE: OBESITY & DIABETES RES: CELL BIOLOGY CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。彭宁顿生物医学研究中心细胞生物学核心设施（PBRC-GCF）的近期目标是为研究人员提供从代谢组织中分离的原代细胞、细胞系和成体干细胞。有了这些试剂、工具和技术，核心机构将扩大研究人员进行与肥胖和糖尿病相关的生理学相关体外研究的范围和机会。核心机构将分离、培养、冷冻保存和分化从人脂肪组织和骨骼肌中获得的原代细胞;此外，将从任何感兴趣的实验动物组织中生成细胞。核心设施将提供使用端粒酶表达载体产生和表征永生化细胞系所需的方法和材料。核心设施的长期目标是为常用的细胞生物学方法和相关的生化测定建立一个机构“记忆”和方案数据库。有了这个信息库，核心设施的工作人员将为需要在细胞生物学领域进行额外培训的新研究人员提供指导。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The immediate goal of the Pennington Biomedical Research Centers Cell Biology Core Facility (PBRC-GCF) is to provide investigators with access to primary cells, cell lines, and adult stem cells isolated from metabolic tissues. With these reagents, tools, and techniques, the Core Facility will expand the scope and opportunity for investigators to conduct physiologically relevant in vitro studies relating to obesity and diabetes. The Core Facility will isolate, culture, cryopreserve, and differentiate primary cells obtained from human adipose tissue and skeletal muscle; in addition, cells will be generated from any experimental animal tissue of interest. The Core Facility will provide the methods and materials required to generate and characterize immortalized cell lines using telomerase expression vectors. The long-term goal of the Core Facility is to establish an institutional "memory" and protocol database for commonly used cell biological methods and related biochemical assays. With this library of information, the Core Facility staff will provide instruction to new investigators who require additional training in the area of cell biol