COBRE: UOK HSC: P2: ANALYSIS OF BIOFILMS ON NATURAL & SYNTHETIC DENTAL SURFACES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。口腔中暴露的牙齿表面（牙釉质和牙骨质）的微生物定殖已被证明与生物膜形成和随后的牙周疾病或龋齿相关。研究表明，与经过抛光或上釉的类似基材相比，具有较高表面粗糙度值的牙齿表面和某些牙科生物材料表面的微生物附着和粘附增加。然而，对减少表面粗糙度或在聚合物基修复生物材料中掺入抗菌单体的初步研究在减少生物膜形成方面的成功有限。本项目的总体目标是确定所选地形和物理性质的效用，作为预测体外生物膜形成的变形链球菌和戈登链球菌。本研究的具体目的是：1）表征牙齿和修复生物材料表面的特定地形和物理特性，并测量S.变异链球菌和S. gordonfi对这些表面的影响，以及2）量化牙齿和修复生物材料表面的激光、化学和机械处理对所选形貌和物理性质的影响，以及S. mutans和S. gordonfi生物膜积累。包括该项目的研究将利用原子力显微镜获得非破坏性的三维形貌和纳米硬度特性的表面测试。接触角测角器将用于测量基材的表面能和润湿性/疏水性，共聚焦激光扫描显微镜将用于定量在体外分批培养装置中形成的生物膜。据推测，天然和合成牙科基质的形貌的准确表征将提供关于这些表面在生物膜发育中的作用的重要基本信息，特别是在牙釉质附近。从该项目中获得的知识也将有助于修改现有牙科修复材料和牙周治疗的地形，或制定新的修复材料和治疗指南。从长远来看，这些知识可以帮助减少与生物膜形成有关的疾病的发生率，从而实现大幅节省成本和改善牙科患者的口腔护理。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microbial colonization of exposed tooth surfaces (enamel and cementum) in the oral cavity has been demonstrated to be associated with biofilm formation and subsequent periodontal disease or dental caries. Studies have demonstrated that tooth surfaces and the surfaces of certain dental biomaterials that have higher surface roughness values have increased microbial attachment and adhesion in comparison with similar substrates that have been polished or glazed. However, initial investigations into the reduction of surface roughness or incorporation of antibacterial monomers in polymer-based restorative biomaterials have had limited success in reducing biofilm formation. The overall goal of this project is to determine the utility of selected topographical and physical properties as predictors of in vitro biofilm formation by Streptococcus mutans and by Streptococcus gordonii. The specific aims of this investigation are: 1) to characterize selected topographical and physical properties of tooth and restorative biomaterial surfaces and to measure biofilm accumulations of S. mutans and S. gordonfi on those surfaces, and 2) to quantify the effect of laser, chemical and mechanical treatments of tooth and restorative biomaterial surfaces on selected topographical and physical properties and S. mutans and S. gordonfi biofilm accumulation. The studies comprising this project will utilize atomic force microscopy to obtain non-destructive 3-dimensional topographical and nanohardness characteristics of the surfaces tested. A contact angle goniometer will be used to measure the surface energy and wettability/hydrophobicity of the substrates, and a confocal laser scanning microscope will be used to quantify the biofilms formed in an in vitro batch culture apparatus. It is hypothesized that accurate characterization of the topography of natural and synthetic dental substrates will provide significant fundamental information on the role of those surfaces in biofilm development, particularly in the proximity of restorations. The knowledge gained from this project will also be useful in the modification of the topography of existing dental restorative materials and periodontal treatments, or in the formulation of guidelines for new restorative materials and treatments. In the long-term, this knowledge could help to reduce the incidence of disease connected with biofilm formation, thereby realizing substantial cost savings and improved oral care for dental patients.