The Ciona savignyi Genetic Map

该补助金是为了响应遗传和基因组计划公告工具而提交的。 新兴模式生物的研究。我建议在海鞘中构建一个300个标记的连锁图谱， 塞维尼玻璃窗，它与将成为注释的高质量基因组参考的内容完全整合 顺序这张图谱不仅有助于克隆突变，而且有助于实现长距离的基因组测序。 C. Savignyi参考序列，其将需要有效地进行全基因组测序， 扫描诱导或自然发生的突变。除了全基因组图谱，高分辨率精细- 将在不确定序列组装的区域中进行标度映射以解决C. savignyi基因组参考序列。这张地图，沿着一个数据库， 分布在整个基因组的多态性标记，将提供给社区。 海鞘是最简单的脊索动物之一，但它们的基本发育机制是 在结构和分子上与脊椎动物的那些保守。尽管保护程度很高， 海鞘基因组的大小与果蝇的（~ 180 Mb）相当，因此在实验中表现出 有利的基因组简单性。此外，海鞘具有许多有吸引力的胚胎学和实验学特性。 这些特征有助于研究发育过程中的基因调控。在过去的几年里，C。萨维尼 已被开发为遗传模式生物，其突变体可容易地从野生种群获得， 通过诱变。遗传图谱、标记和基因组组装的长距离邻接是 缺失的，至关重要的，建立C的资源。savignyi作为一个成熟的遗传和基因组 模式生物 塞维尼玻璃海鞘和它的姐妹种，玻璃海鞘，是唯一的信息方面， 脊索动物胚胎发育的机制。胚胎的基本特征和具体特征 发育和基因调控在人类和这些脊索动物之间是保守的。洞察 以玻璃海鞘为模型的调节和发育的分子机制非常有效， 与人类的同源过程有关。

This grant is submitted in response to the Program Announcement Tools for Genetic and Genomic Studies in Emerging Model Organisms. I propose to construct a 300-marker linkage map in the ascidian, Ciona savignyi, that is fully integrated with what will become the annotated high quality genome reference sequence. This map will not only aid in the cloning of mutations, but also in achieving the long range contiguity in the C. savignyi reference sequence that will be needed to efficiently perform whole genome scans for induced or naturally occurring mutations. In addition to the genome-wide map, high-resolution fine- scale mapping in regions of uncertain sequence assembly will be performed to resolve ambiguities in the C. savignyi genome reference sequence. The map, along with a database of thousands of potentially polymorphic markers distributed across the genome, will be made available to the community. Ascidians are among the simplest chordates, yet their fundamental developmental mechanisms are structurally and molecularly conserved with those of vertebrates. Despite the high level of conservation, ascidian genomes are comparable in size to Drosophila's (-180 Mb), thus exhibiting experimentally advantageous genomic simplicity. Further, ascidians have many attractive embryological and experimental features that facilitate studies of gene regulation during development. Over the past few years, C. savignyi has been developed as a genetic model organism, with mutants readily obtainable from wild populations or by mutagenesis. The genetic map,the markers, and the long-range contiguity of the genome assembly are the missing, critically important, resources to establish C. savignyi as a full-fledged genetic and genomic model organism. Ciona savignyi and its sister species, Ciona intestinalis, are uniquely informative with regards to the mechanisms of chordate embryonic development. Fundamental as well as specific features of embryonic development and gene regulation are conserved between humans and these chordates. Insights into the molecular mechanisms of regulation and development using Ciona as a model are highly effective and relevant to the homologous processes in human.