GENE LIBRARY RESOURCE FOR THE SEA URCHIN S PURPURATUS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。描述（由申请人提供）：本提案寻求对紫海胆（Strongylocentrotus purpuratus）阵列基因文库资源的支持。该资源是全基因组测序工作的基因组材料的唯一供应商，除了其服务于细胞和发育研究界。资源有两个部分：一个机器人阵列库和一个计算设施。机器人设施生成，维护和分发阵列图书馆资料的研究界。新的服务目标是网页，以提高系统的有用性。这些页面将详细说明：1）干扰试剂、探针和该系统中使用的特异性基因引物; 2）海胆转录因子的表达和功能; 3）海胆DNA序列和资源质粒克隆之间的链接; 4）实现特定软件和文档的分发;以及5）列出社区特定信息。在研究部分，我们将提供cDNA文库，为基因组的注释提供信息。我们计划：1）从成人桡神经产生组织特异性文库; 2）产生含有成人骨骼发生基因的文库; 3）构建含有成人口腔结构分化基因的文库; 4）从青少年灯笼产生肌肉基因的文库;和5）为了确定现有成人组织库的质量，我们设想海胆基因组资源作为分布式系统的一部分，组成了一个高度关联的可用的全基因组资源。我们作为一个门户网站，将实验发育生物学积累的经验带给生物信息学家。同时，我们也为实验者提供了生物信息学工具和基因组序列数据库。上面详述的所有个人目标都是对这一观点的直接回应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal seeks support for the Arrayed Gene Library Resource for the purple sea urchin, Strongylocentrotus purpuratus. This Resource is the sole provider of genomic materials for the whole genome sequencing effort in addition to its service to the cell and developmental research community. There are two parts to the Resource: a robotic arrayed library and a computational facility. The robotics facility generates, maintains and distributes arrayed library materials to the research community. The new service aims are web pages to enhance the usefulness of the system. The pages will detail: 1) perturbing reagents, probes, and specific gene primers used in this system; 2) expression and function of sea urchin transcription factors; 3) links between sea urchin DMA sequences and resource plasmid clones; 4) implement distribution of specific software and documentation; and 5) list community specific information. In the research component we will provide cDNA libraries to inform the annotation of the genome. We plan to: 1) to produce a tissue-specific library from adult radial nerve; 2) to produce a library containing adult skeletogenic genes; 3) to construct a library containing differentiation genes for adult oral structures; 4) to produce a library from the juvenile lantern for muscle genes; and 5) to determine the quality of existing adult tissue libraries We envision the Sea Urchin Genome Resource as part of a distributed system whose individual efforts make up a highly connected, useable whole genome resource. We act as a portal to bring the accumulated experience of experimental developmental biology to bioinformaticists. And we present the bioinformatics tools and databases of genomic sequence collections to the experimentalist. All of the individual goals detailed above are direct responses to this view.