CORE--ADVANCED TECHNOLOGY LABORATORIES

所有抗感染药物的临床试验都使用了微生物学。 评价化疗药物体内活性的试验 学习。抗逆转录病毒药物的临床试验也需要类似的测试。 UCSF ACTU病毒学核心实验室的目标是进行 已知或怀疑测量体内抗逆转录病毒效果的试验 可重现，可进行已知或怀疑在体内测量的测试 以可重复、快速和准确的方式发挥抗逆转录病毒作用。 目前，最广泛用于评估体内抗逆转录病毒的测试 影响因素为血清HIVp24(核心)抗原和外周血细胞培养 血液中的单个核细胞检测艾滋病毒。尽管血清中的变化 P24抗原的浓度似乎是抗逆转录病毒的一个很好的指标。 逆转录酶抑制剂对白细胞简易培养的影响 因为艾滋病毒并没有。因此，ACTG正在走向量化文化 血浆作为体内抗逆转录病毒作用的另一个标志，以及其他 测试(例如，在聚合酶链式反应扩增后测量艾滋病毒的cdna或基因组RNA) 正在考虑中。加州大学旧金山分校ACTU核心病毒学实验室 自1987年开始进行艾滋病毒培养和p24检测，并一直 自1989年以来，连续获得p24抗原检测和艾滋病毒认证 自1990年以来的文化。此外，到目前为止，我们在一些方面还有经验 未经认证的程序，如细胞的定量微培养和 血浆，以及HIV基因和基因组RNA的聚合酶链式反应定量。具体的 加州大学旧金山分校ACTU核心病毒学实验室的目标是进行艾滋病毒培养， P24抗原定量，以及ACTU所需的其他病毒学测试 为了以后的测试；通过参加ACTG保持我们的熟练程度 质量保证计划，并与ACTG在全国范围内合作开发 并验证体内抗逆转录病毒效果的其他标志物。

All clinical trials of anti-infective agents have employed microbiologic tests to assess the in vivo activity of the chemotherapeutic agent under study. Similar tests are needed for clinical trails of antiretrovirals. It is the goal of the Virology Core Laboratory of the UCSF ACTU to perform tests known or suspected of measuring in vivo antiretroviral effect in a reproducible, perform tests known or suspected of measuring in vivo antiretroviral effect in a reproducible, rapid and accurate fashion. Currently the tests most widely used for assessing in vivo antiretroviral effect are the serum HIV p24 (core) antigen, and culture of peripheral blood mononuclear cells for HIV. Although changes in the serum concentration of p24 antigen appear to be a good measure of antiretroviral effect with reverse transcriptase inhibitors, simple culture of leukocytes for HIV has not. The ACTG is therefore moving toward quantitative culture of plasma as another marker of in vivo antiretroviral effect, and other tests (e.g. measurement of HIV cDNA or genomic RNA after PCR amplification) are being considered. The UCSF ACTU Core Virology Laboratory has been performing HIV culture and p24 determination since 1987, and has been certified continuously for p24 antigen testing since 1989 and for HIV culture since 1990. In addition, we have experience in some as-yet uncertified procedures such as quantitative microculture of cells and plasma, and PCR quantitation of HIV cDNA and genomic RNA. The specific aims of the UCSF ACTU Core Virology Laboratory are to perform HIV cultures, p24 antigen quantitation, and other virologic testing as needed for ACTU's for later testing; to maintain our proficiency by participating in ACTG quality assurance programs, and to work with the ACTG nationally to develop and validate other markers of in vivo antiretroviral effects.