SeaScreen: expanding a bioprocess repertoire by smart marine microbe screening.

本提案的目的是发现具有工业相关底物特异性的新氧化酶，用于与Ingenza脱羧生物工艺相结合，用于制造对映纯手性胺和氨基酸。目前，由于缺乏合适的氧化酶，该技术不能用于(R)-胺或l -氨基酸家族的大多数底物，因此无法进入重要的市场。海洋微生物是一种巨大的、多样的、不发达的新型生物催化酶的来源。该项目首次汇集了海洋生物技术和生物催化领域的重要成员，并将通过开发高度创新和强大的筛选和选择技术以及新颖，高产，可扩展和经济的酶生产系统，提供工业上有用的新型氧化酶。

The aim of this proposal is to discover new oxidase enzymes with industrially relevant substrate specificities for integration with the Ingenza deracemisation bioprocess for the manufacture of enantiopure chiral amines and amino acids. Currently, the technology cannot be used on the majority of substrates in the (R)-amine or L-amino acid families due to the lack of suitable oxidases and significant markets are therefore inaccessible. Marine microorganisms represent a vast, diverse and underdeveloped source of novel enzymes for biocatalysis. This project brings together significant members of the fields of marine biotechnology and biocatlaysis for the first time and will deliver industrially useful novel oxidases by developing highly innovative and powerful screening and selection technologies and novel, high yielding, scalable and economic enzyme production systems.