Succinic Esters from Renewable Feedstocks (SERF)

工作包B1: 1-2年（IGER， FA）乳酸及其衍生物的生产。乳酸菌将用于乳酸及其衍生物的生产。目前，糖化合物的乳酸生产受到缺乏生物耐受性的限制，导致中毒和产量低。已经确定了新的更具耐受性的乳酸菌菌株，并将与现有的和新的分离株一起对这些菌株进行评估。该工作包将利用新的和现有的乳酸菌菌株优化乳酸生产。方法还将寻求通过利用来自残余植物生物量的不同馏分来加强净化。工作包B2：第1-2年（IGER）。琥珀酸及其衍生物的生产。瘤胃微生物已经在动物营养方面进行了广泛的研究，但尚未在工业应用的拟议背景下进行研究，见附件B技术壁垒。IGER将为新的应用开发现有的技术。几种瘤胃微生物产生琥珀酸盐作为初级发酵产物。琥珀酸原纤维杆菌和黄瘤球菌的混合物将用于琥珀酸的生产，并优化发酵。工作包B3 1-2年（IGER）糖基化合物的生产。在本工作包中，将使用细胞壁降解酶，如新型纤维素酶混合物、内切木聚糖酶、阿魏酸酯酶对未加工、机械分离和果汁/纤维部分进行处理，以确定转化效率与加工水平的关系。这一评估将通过确定获得最大糖产量的最低加工水平来提高经济效益。此步骤将与工作包B1和B2集成。开发阶段C：将平台化学品转化为化工原料。在工作包B1、B2和B3中开发的平台分子将通过一系列常规和绿色合成路线转化为化学原料。工作将在生物复合材料中心的一个新的50升设施上进行中试规模生产之前，使用反应量热法评估该过程。然后，工业合作伙伴将在选定的应用（树脂，涂料，塑料和化妆品）中验证产品。目标分子的实验室规模生产。将进行实验室研究（RC1），以评估平台化学品转化为化学原料，如乳酸转化为乳酸乙酯和丙烯酸。还将研究琥珀酸转化为琥珀酸酯、四氢呋喃、1,4丁二醇和戊二醇的其他反应。工作包C2 2-3年。中试规模生产及产品配方。在适当情况下，财团伙伴将利用工作包中制定的议定书进行化学原料的中试规模生产。合作伙伴生产的化合物将包括聚乳酸、多元醇、多元醇酯、琥珀酸酯。

Work Package B1: Year 1-2 (IGER, FA) Production of lactic acid and its derivatives. Lactobacilli will be studied for the production of lactic acid and its derivatives. Currently lactic acid production from sugar compounds is limited by the lack of bio-tolerance which results in poisoning and low yields. New more tolerant strains of lactobacillus have been identified and these will be assessed, alongside existing and new isolates. The work package will optimise lactic acid production using novel and existing strains of lactobacilli. Methods will also seek to enhance purification by utilising different fractions from residual plant biomass. Work Package B2: Year 1-2 (IGER). Production of succinic acid and its derivatives. Rumen microbes have been studied extensively in animal nutrition but have yet to be studied in this proposed context for industrial applications, see annex B technology barriers. IGER will develop this existing know-how for new applications. Several rumen microbes produce succinate as a primary fermentation product. A cocktail of Fibrobacter succinogenes and Ruminococcus flavefaciens will be used for the production of succinic acid and optimised for fermentation. Work Package B3 Year 1-2 (IGER) Production of sugar based compounds. In this work package the use of cell wall degrading enzymes e.g. novel cellulase mixtures, endo xylanases, ferulic acid esterase on unprocessed, mechanically separated and juice/ fibre fractions will be undertaken to identify conversion efficiency vs. level of processing. This assessment will improve the economics by identifying the minimal level of processing for maximal yield of sugar. This step will be integrated with work packages B1 and B2. Development Phase C: Chemical conversions of platform chemicals to chemical feedstocks. Platform molecules developed in work packages B1, B2 and B3 will be converted into chemical feedstocks using a range of conventional and green synthesis routes. Work will assess the process using reaction calorimetry before pilot scale production on a new 50 litre facility at the BioComposites Centre. Industrial partners will then validate products in selected applications (resins, coatings, plastics and cosmetics) Work Package C1 Year 2-3 (BC). Lab scale production of target molecules. Laboratory studies (RC1) will be undertaken to assess the conversion of platform chemicals to chemical feedstocks such as lactic acid to ethyl lactate and acrylic acid. Other conversions of succinic acid to succinic esters, tetrahydrofuran, 1,4 butanediol and pentanediol will also be studied. Work Package C2 Year 2-3. Pilot scale production and product formulation. Where appropriate consortium partners will undertake pilot scale production of chemical feedstocks using the protocols developed in the work packages. Compounds made by the partners will include PLA, polyols, polyol esters, succinate esters.