Intravital Imaging, Invasive Cell Collection and Expression Profiling

核心A将提供所需的专业知识、设备和解释技能， 在体内的分辨率，肿瘤细胞的侵袭表型，其相应的表达谱， FISH，以及肿瘤细胞和基质细胞（包括巨噬细胞）之间相互作用的表征， 都在原发肿瘤内和肺转移部位。此核心是制作的唯一实用方法 多光子成像，以及从活肿瘤中相关收集侵袭性细胞，可供所有 由于专门知识和设备是独特的、昂贵的，而且不能重复供个人使用，因此， 多光子成像和侵入性细胞收集所需的技能使得这些方法成为必要。 由相同的人对所有项目进行实践，以确保结果的一致性，以便可以比较结果 在动物模型中。核心A还将作为表达谱分析和生物信息学的联络点， AECOM和AECC设施使这些技术在项目中的应用统一。最后， 核心A将支持使用高分辨率FISH进行原位表达谱分析。 核心A专用的设施和设备为： 1.活体肿瘤细胞和基质细胞的多光子显微镜观察 2.用于收集活的侵袭性肿瘤细胞和基质细胞的体内侵袭测定和研究 自分泌和旁分泌刺激入侵 3.使用抗体和PCR的侵入性细胞分型 4. FACs和micro-FACs 5. RNA扩增、表达谱分析和生物信息学 6. FISH显微术与相关免疫荧光

Core A will provide the expertise, equipment and interpretive skills needed to define, at cellular resolution in vivo, the invasive phenotype of tumor cells, their corresponding expression profiles, correlated FISH, and the characterization of interactions between tumor cells and stromal cells including macrophages, all within the primary tumor and at sites of lung metastasis. This Core is the only practical way to make multiphoton imaging, and the correlated collection of invasive cells from live tumors, available to all of the projects since the expertise and equipment is unique, expensive, and cannot be duplicated for individual use. The skill required for multiphoton imaging and invasive cell collection necessitates that these methods be practiced by the same people for all projects to ensure uniformity of results so that results can be compared across animal models. Core A will also act as the liaison for expression profiling and bioinformatics with the AECOM and AECC facilities making the application of these technologies across projects uniform. Finally, Core A will support the use of high resolution FISH for in situ expression profiling. The Facilities and equipment to be dedicated to Core A are: 1. Multiphoton microscopy of tumor cells and stromal cells in vivo 2. In vivo Invasion Assay for the collection of live invasive tumor cells and stromal cells and investigation of autocrine and paracrine stimulation of invasion 3. Invasive cell typing using antibodies and PCR 4. FACs and micro-FACs 5. RNA amplification, expression profiling and Bioinformatics 6. FISH microscopy with correlated immunofluorescence