Signaling and Motility Assays

核心组件B，信令和动力分析核心，将提供专业知识、协议、分析 以及对检查个体行为的体外试验的解释，以及肿瘤和 基质细胞。核心执行检测二维可移动细胞行为的分析，三维 侵袭、血管生成和跨内皮细胞迁移。这些化验可以进行全面的检查。 在趋化过程中的细胞行为，并提供快速和完整的侵袭能力的分析 在昂贵和耗时的动物研究之前，一种给定的细胞类型。此外，这些体外检测支持 并补充了活体成像、侵入性细胞收集和 表情分析核心(核心A)。核心B还将作为使用独特蛋白质生物传感器的联络人 以及用于检查英托波德形成的分析，以使这些技术的应用是一致的 在这些项目中。由Core B支持的体外检测提供了急需的信息 肿瘤细胞侵袭和血管内侵袭过程中的细胞行为和机制 癌症患者是指肿瘤细胞离开原发肿瘤，传播到身体内较远部位的能力 并形成继发性肿瘤。该核心提供的分析专门检查个人行为 以及肿瘤与基质细胞的相互作用，将为理解其机制提供基础。 调节肿瘤细胞的运动和侵袭。

Core Component B, the Signaling and Motility Assays Core, will provide the expertise, protocols, analysis and interpretation of in vitro assays that examine the individual behavior, and interaction of tumor and stromal cells. The Core performs assays examining two-dimensional motile cell behavior, three-dimensional invasion, angiogenesis and transendothelial migration. These assays permit a comprehensive examination of cell behavior during chemotaxis, and provide a rapid and complete analysis of the invasive capabilities of a given cell type prior to costly and time-consuming animal studies. Moreover these in vitro assays support and complement the in vivo studies provided by the Intravital Imaging, Invasive Cell Collection and Expression Profiling Core (Core A). Core B will also act as a liaison for the use of unique protein biosensors and for assays examining invadopod formation so that the application of these technologies is uniform amongst the projects. The in vitro assays supported by Core B provide much needed information about cellular behavior and mechanism during tumor cell invasion and intravasation.The leading cause of death in cancer patients is the ability of tumor cells to leave the primary tumor, travel to distant sites within the body and form secondary tumors. The assays provided by this Core specifically examine the individual behavior and interaction of tumor and stromal cells, and will provide the foundation for understanding the mechanisms regulating tumor cell motility and invasion.